Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (<Emphasis Type="Italic">Arachis</Emphasis><Emphasis Type="Italic">hypogaea</Emphasis> L.) genotypes |
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Authors: | Faqian Xiong Ruichun Zhong Zhuqiang Han Jing Jiang Liangqiong He Weijian Zhuang Ronghua Tang |
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Institution: | (1) Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China;(2) Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China;(3) Fujian Province Key Lab of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; |
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Abstract: | Cultivated peanut possesses an extremely narrow genetic basis. Polymorphism is considerably difficult to identify with the
use of conventional biochemical and molecular tools. For the purpose of obtaining considerable DNA polymorphisms and fingerprinting
cultivated peanut genotypes in a convenient manner, start codon targeted polymorphism technique was used to study genetic
diversity and relatedness among 20 accessions of four major botanical varieties of peanut. Of 36 primers screened, 18 primers
could produce unambiguous and reproducible bands. All 18 primers generated a total of 157 fragments, with a mean of 8.72 ranging
from 4 to 17 per primer. Of 157 bands, 60 (38.22%) were polymorphic. One to seven polymorphic bands were amplified per primer,
with 3.33 polymorphic bands on average. Polymorphism per primer ranged from 14.29 to 66.67%, with an average of 36.76%. The
results revealed that not all accessions of the same variety were grouped together and high genetic similarity was detected
among the tested genotypes based on cluster analysis and genetic distance analysis, respectively. Further, accession-specific
markers were observed in several accessions. All these results demonstrated the following: (1) start codon targeted polymorphism
technique can be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut, and (2) it possesses
considerable potential for studying genetic diversity and relationships among peanut accessions. |
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