Osteogenic differentiation of human periosteal-derived cells in a three-dimensional collagen scaffold |
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Authors: | Young-Mo Ryu Young-Sool Hah Bong-Wook Park Deok Ryong Kim Gu Seob Roh Jong-Ryoul Kim Uk-Kyu Kim Gyu-Jin Rho Geun-Ho Maeng June-Ho Byun |
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Institution: | (1) Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical Center (BK21), Gyeongsang National University School of Medicine, Jinju, 660-702, Republic of Korea;(2) Clinical Research Institutue of Gyeongsang National University Hospital, Jinju, Republic of Korea;(3) Department of Biochemistry, Institute of Health Sciences, Biomedical Center (BK21), Gyeongsang National University School of Medicine, Jinju, Republic of Korea;(4) Department of Anatomy, Institute of Health Sciences, Biomedical Center (BK21), Gyeongsang National University School of Medicine, Jinju, Republic of Korea;(5) Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Busan, Republic of Korea;(6) College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea |
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Abstract: | This study examined the osteogenic differentiation of cultured human periosteal-derived cells grown in a three dimensional
collagen-based scaffold. Periosteal explants with the appropriate dimensions were harvested from the mandible during surgical
extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the
cells were divided into two groups and cultured for 28 days. In one group, the cells were cultured in two-dimensional culture
dishes with osteogenic inductive medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In the other group,
the cells were seeded onto a three-dimensional collagen scaffold and cultured under the same conditions. We examined the bioactivity
of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and measurements of the calcium content in the
periosteal-derived cells of two groups. Periosteal-derived cells were successfully differentiated into osteoblasts in the
collagen-based scaffold. The ALP activity in the periosteal-derived cells was appreciably higher in the three-dimensional
collagen scaffolds than in the two-dimensional culture dishes. The levels of ALP and osteocalcin mRNA in the periosteal-derived
cells was also higher in the three-dimensional collagen scaffolds than in the two-dimensional culture dishes. The calcium
level in the periosteal-derived cells seeded onto three-dimensional collagen scaffolds showed a 5.92-fold increase on day
7, 3.28-fold increase on day 14, 4.15-fold increase on day 21, and 2.91-fold increase on day 28, respectively, compared with
that observed in two-dimensional culture dishes. These results suggest that periosteal-derived cells have good osteogenic
capacity in a three-dimensional collagen scaffold, which provides a suitable environment for the osteoblastic differentiation
of these cells. |
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