Plasmids in carboxydotrophic bacteria: physical and restriction analysis |
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Authors: | Maria Kraut Ortwin Meyer |
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Institution: | (1) Institut für Pflanzenphysiologie, Zellbiologie und Mikrobiologie, Freie Universität Berlin, Königin-Luise-Strasse 12-16a, D-1000 Berlin;(2) Present address: Lehrstuhl für Mikrobiologie, Universität Bayreuth, Postfach 101251, D-8580 Bayreuth, FRG |
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Abstract: | Twenty species and strains of aerobic CO-oxidizing bacteria were screened for the occurrence of plasmids. Six of them harbored plasmids between 45 and 558kb. Megaplasmids of 428 and 558 kb were resolved in Alcaligenes carboxydus. Restriction digest patterns of plasmids from different carboxydotrophic bacteria were dissimilar. However, the patterns obtained with the plasmids from the strains OM5, OM4 and OM2 of Pseudomonas carboxydovorans were very much the same. The nine cured mutants of P. carboxydovorans OM5, as well as the deletion mutant OM5-29, could not grow chemolithotrophically with CO or H2 plus CO2, as they were devoid of CO dehydrogenase, hydrogenase and ribulose bisphosphate carboxylase. The deletion mutant OM5-24 retained the ability to grow with CO. It could not grow with H2 plus CO2 and was devoid of H2ase. The data suggest the residence of structural and/or regulatory genes of CODH, H2ase and RuBPCx on plasmid pHCG3 of P. carboxydovorans.Abbreviations CODH
carbon monoxide dehydrogenase
- CRM
cross reacting material
- EMS
ethyl methane sulfonate
- H2ase
hydrogenase
- kb
kilobase
- NTG
N-methyl-N-nitro-N-nitrosoguanidine
- RuBPCx
ribulose bisphosphate carboxylase
- SDS
sodium dodecylsulfate |
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Keywords: | Carbon monoxide Autotrophic bacteria Carboxydotrophic bacteria Plasmids Restriction analysis Mutants Deletion |
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