Phylogeny and molecular fingerprinting of green sulfur bacteria |
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Authors: | J Overmann Christian Tuschak |
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Institution: | Pal?omikrobiologie, Institut für Chemie und Biologie des Meeres, Postfach 2503, D-26111 Oldenburg, Germany Tel. +49-441-970-6376; Fax +49-441-798-3583 e-mail j.overmann@palmikro.icbm.uni-oldenburg.de, DE
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Abstract: | The 16S rDNA sequences of nine strains of green sulfur bacteria (Chlorobiaceae) were determined and compared to the four
known sequences of Chlorobiaceae and to sequences representative for all eubacterial phyla. The sequences of the Chlorobiaceae
strains were consistent with the secondary structure model proposed earlier for Chlorobium vibrioforme strain 6030. Similarity values > 90.1% and Knuc values < 0.11 indicate a close phylogenetic relatedness among the green sulfur bacteria. As a group, these bacteria represent
an isolated branch within the eubacterial radiation. In Chlorobiaceae, a similar morphology does not always reflect a close
phylogenetic relatedness. While ternary fission is a morphological trait of phylogenetic significance, gas vesicle formation
occurs also in distantly related species. Pigment composition is not an indicator of phylogenetic relatedness since very closely
related species contain different bacteriochlorophylls and carotenoids. Two different molecular fingerprinting techniques
for the rapid differentiation of Chlorobiaceae species were investigated. The 16S rDNA fragments of several species could
not be separated by denaturing gradient gel electrophoresis. In contrast, all strains investigated during the present work
gave distinct banding patterns when dispersed repetitive DNA sequences were used as targets in PCR. The latter technique is,
therefore, well suited for the rapid screening of isolated pure cultures of green sulfur bacteria.
Received: 26 August 1996 / Accepted: 8 January 1997 |
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Keywords: | Green sulfur bacteria Chlorobiaceae DNA sequences Ribosomal RNA Phylogeny Taxonomy |
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