The effect of carbon dioxide on the growth kinetics of fructose-limited chemostat cultures of Acetobacterium woodii DSM 1030 |
| |
Authors: | Andrew R Godley Paul E Linnett John P Robinson |
| |
Institution: | (1) Shell Research Limited, Sittingbourne Research Centre, ME9 8AG Sittingbourne, Kent, UK;(2) School of Biological Science, Queen Mary and Westfield College, Mile End Road, E1 4NS London, UK |
| |
Abstract: | The growth of the anaerobic acetogenic bacterium Acetobacterium woodii DSM 1030 was investigated in fructose-limited chemostat cultures. A defined medium was developed which contained fructose, mineral salts, cysteine · HCl and Ca pantothenate (1 mg · 1–1) supplied in a vitamin supplement. Growth at high dilution rates was dependent on the presence of CO2 in the gas phase. The
max was found to be 0.16 h–1 and the fructose maintenance requirement was 0.1 to 0.13 mmol fructose · (g dry wt)–1 · h–1. A growth yield of 61 g dry wt · (mol fructose)–1, corrected for the cell maintenance requirement and for incorporation of fructose carbon into cell biomass, was determined from the fructose consumption. A corresponding growth yield of 69 g dry wt · (mol fructose)–1 was calculated from the acetate production assuming that fructose fermentation was homoacetogenic. A YATP of 12.2 to 13.8 g dry wt · (mol ATP)–1 was calculated from these growth yields using a value of 5 mol ATP · (mol fructose)–1 as an estimate of the amount of ATP synthesised from fructose fermentation. The addition of yeast extract (0.5 g · 1–1) to the medium did not influence the
max or cell yield. After prolonged growth under fructose-limited conditions the requirement of the culture for CO2 in the gas phase was reduced.Abbreviations YE
yeast extract
- IC
inorganic carbon
- D
fermenter dilution rate : h–1
- MX
maintenance requirement for X: mmol X · (g dry wt)–1 · h–1
- X
may be fructose (Fruct), fructose consumed in energy metabolism (Fruct E]), acetate (Ac)
- ATP
CO2, NH
inf4
sup+
or Pi
- qX
specific rate of utilisation or consumption of X: mmol X · (g dry wt)–1 · h–1
- V
fermenter volume: litre
- rC
· Cell, fermenter cell carbon production: mmol C · h–1
- YX
yield of cells on X: g dry wt · (mol X)–1
- Y
infx
supmax
the yield corrected for cell maintenance: g dry wt · (mol X)–1
- SATP
stoichiometry of ATP synthesis from fructose: mol ATP · (mol frucose)–1
- x
cell concentration: g dry wt · 1–1
-
specific growth rate : h–1
-
max
maximum specific growth rate: h–1 |
| |
Keywords: | Acetobacterium woodii Fructose growth yields Chemostat culture Yeast extract Carbon dioxide YATP |
本文献已被 SpringerLink 等数据库收录! |
|