Characterization of the cell wall of the unicellular cyanobacterium Synechocystis PCC 6714 |
| |
Authors: | Uwe J Jürgens Jochen R Golecki Jürgen Weckesser |
| |
Institution: | (1) Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, D-7800 Freiburg, Federal Republic of Germany |
| |
Abstract: | Cell walls free of cytoplasmic- and thylakoid membranes were isolated from Synechocystis PCC 6714 by sucrose density gradient centrifugation and extraction with Triton X-100. The Triton-insoluble cell wall fraction retained the multilayered fine structure. Peptidoglycan, proteins, polysaccharides, lipopolysaccharides, lipids and carotenoids were found as constituents of the cell wall. Polypeptide and lipid patterns of cell walls were completely different from that of the cytoplasmic/thylakoid membrane fraction. The purified cell walls contained about twelve outer membrane proteins. The two major polypeptides (Mr 67,000 and 61,000) were found to be associated with the peptidoglycan by ionic interactions.Myxoxanthophyll (major carotenoid), related carotenoid-glycosides and zeaxanthin were the predominating carotenoids of the cell wall of Synechocystis PCC 6714 over echinenone and -carotene. A polar unknown carotenoid was observed, the absorption spectrum of which resembled that of myxoxanthophyll. It was exclusively found in cell walls, but not in the cytoplasmic/thylakoid membrane fraction.Abbreviations Hep
heptose
- DGDG
digalactosyldiglyceride
- MGDG
monogalactosyldiglyceride
- SL
sulfolipid
- PC
phosphatidylcholin
- PG
phosphatidylglyceride
Dedicated to Prof. Dr. G. Drews on the occasion of his 60th birthday |
| |
Keywords: | Carotenoids Cell wall Cyanobacterium Peptidoglycan-associated proteins Polypeptide pattern Synechocystis |
本文献已被 SpringerLink 等数据库收录! |
|