首页 | 本学科首页   官方微博 | 高级检索  
   检索      

热休克诱导虹鳟四倍体
引用本文:马涛,朱才宝,朱秉仁.热休克诱导虹鳟四倍体[J].水生生物学报,1987,11(4):329-336,i008.
作者姓名:马涛  朱才宝  朱秉仁
作者单位:[1]山西大学生物系,太原 [2]山西省虹鳟鱼实验场,朔县
摘    要:虹鳟卵受精后5—9小时期间,用热休克处理,12月龄时检查,四倍体出现频率为5%。较高的温度处理可导致卵的高死亡率。2n=60的虹鳟核型中,有中部和亚中部着丝点染色体22对,近端着丝点染色体1对,端部着丝点染色体7对,总臂数NF=104。4n=120的虹鳟四倍体核型中,有22套中部和亚中部着丝点染色体,1套近端着丝点染色体,和7套端部着丝点染色体,总臂数NF=208。未发现有染色体倍性镶嵌的个体。分析比较了二倍体和四倍体两类鱼的红细胞及其核的9个度量值(DNA相对含量,细胞及核的长轴、短轴,面积和体积),为应用红细胞鉴定四倍体虹鳟提供了倍性标准。在形态、解剖和生长速度方面未发现两类鱼有什么差别。

关 键 词:四倍体  虹鳟  着丝点  染色体  热休克  月龄  倍性  NF  红细胞  诱导

TETRAPLOID OF RAINBOW TROUT (SALMO GAIRDNERI RICHARDSON) INDUCED BY HEAT SHOCKS
Ma Tao Zhu Caibao.TETRAPLOID OF RAINBOW TROUT (SALMO GAIRDNERI RICHARDSON) INDUCED BY HEAT SHOCKS[J].Acta Hydrobiologica Sinica,1987,11(4):329-336,i008.
Authors:Ma Tao Zhu Caibao
Abstract:Heat shocks were applied to rainbow trout eggs, starting between 5h and 9h after fertili-zation. The frequency of occurrence of tetraploid was 5% in fish of twelve-month-old. During. the experiment, the higher temperature resulted in high mortality rate of fish eggs.Being different from the diploid rainbow trout which consists of 22 pairs of metacentric and submetacentric chromosomes, 1 pair of subtelocentric chromosomes and 7 pairs of telocentric chromosomes, the karyotype of the tetraploid rainbow trout consists of 22 sets of metacentric and submetacentrie chromosomes, 1 set of subtelocentric chromosomes and 7 sets of telocentric chromosomes. No mosaic polyploids were found amongst the young troutNine erythrocyte indexes (DNA content, and major axis, minor axis, surface area and volume of cell and nucleus) of diploid and tetraploid rainbow trout were analysed and compared in order to provide the basis of identifying tetraploid in rainbow trout by means of erythrocyte inspection. No differences were found between the tetraploid and the diploid in morphology, anatomy, and growth rate.
Keywords:Rainbow trout  heat shock  tetraploid
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号