| 筛选冠突伪尾柱虫有小核细胞系和无小核细胞系饥饿期差异表达的ESTs |
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| 引用本文： | 刘星吟,毛永真,李甘霖,金立培.筛选冠突伪尾柱虫有小核细胞系和无小核细胞系饥饿期差异表达的ESTs[J].水生生物学报,2006,30(3):333-338. |
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| 作者姓名： | 刘星吟 毛永真 李甘霖 金立培 |
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| 作者单位： | 1. 中山大学生命科学学院,广州,510275
2. 香港理工大学应用生化系 |
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| 基金项目： | 国家自然科学基金(No.30370210)资助 |
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| 摘 要： | 利用显微切割的方法建立大型腹毛目纤毛虫---冠突伪尾柱虫(Pseudourostyla cristata)的无小核细胞系,分别提取有小核和无小核细胞系的总RNA,用SMART-PCR方法直接合成dscDNA,进而运用抑制性消减杂交技术(Sup-pression subtractive hybridization,SSH)对冠突伪尾柱虫去小核前后的差异表达基因进行研究,构建了有与无小核细胞系在饥饿期差异表达基因的消减文库,随机挑取了284个克隆,应用cDNA阵列技术鉴定阳性克隆。将鉴定出的17个阳性克隆进行Blastx分析,结果表明17个EST(Expressed sequence tag)均可能与小核体功能密切相关,此为进一步阐明小核的遗传机理奠定了基础。
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| 关 键 词： | 抑制性消减杂交 差异表达基因 冠突伪尾柱虫 大核 小核 |
| 文章编号： | 1000-3207(2006)03-0333-06 |
| 收稿时间： | 2004-10-09 |
| 修稿时间： | 2006-01-20 |
Identification of ESTs expressed differentially between micronucleates and amicronucleates of hypotri-chous ciliate, Pseudourostyla cristata during starvation |
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| LIU Xing-Yin,MAO Yong-Zhen,LI Gan-Lin,JIN Li-Pei.Identification of ESTs expressed differentially between micronucleates and amicronucleates of hypotri-chous ciliate, Pseudourostyla cristata during starvation[J].Acta Hydrobiologica Sinica,2006,30(3):333-338. |
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| Authors: | LIU Xing-Yin MAO Yong-Zhen LI Gan-Lin JIN Li-Pei |
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| Institution: | 1. Life Science School, Zhongshan University, Guangzhou 510275; 2. Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University |
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| Abstract: | |
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| Keywords: | Suppression subtractive hybridization Differentially expressed genes Pseudourostyla cristata Macronuclei Micronuclei |
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