绿色荧光蛋白标记的嗜水气单胞菌在温暖水体的存活及稳定性

用绿色荧光蛋白基因(GFP)标记嗜水气单胞菌4332株(Ah4332GFP),检测其在温暖水体中的存活及稳定性.在灭菌自然塘水中,Ah4332GFP存活47d之久,该菌浓度随时间而变化,呈现两个峰值;在非灭菌自然塘水中,Ah4332GFP存活18d,该菌浓度均随时间延长呈下降趋势.表明Ah4332GFP和天然细菌之间的相互作用影响其稳定性.用M9培养基检测Ah4332GFP质粒的稳定性,结果显示在传代培养40、70代后,质粒稳定率分别保持在473%和205%.可见pGFP是一种相对稳定的质粒,Ah4332GFP可用于微生物生态的研究.  

SURVIVAL AND STABILITY OF GREEN FLUORESCENT PROTEIN MARKED (GFP-MARKED) AEROMONAS HYDROPHILA IN WARM WATER

The survival and stability of Aeromonas hydrophila (Ah) strain 4332 GFP marked with green fluorescent protein plasmid (pGFP) in the warm water was tested. The results showed that Ah4332 GFP survived 47 d and the number of green fluorescent cells (GFCs) had two peaks in the autoclaved pond water. In the natural pond water, Ah4332 GFP survived 18 d, but the number of GFCs declined during the experimental period. It suggested that Ah4332 GFP and the natural bacteria in the pond water interacted and affected the stability of marked bacteria. In the medium M9, Ah4332 GFP strain could culture to 40 and 70 generations and the rates of stability of pGFP were 47.3% and 20.5% , respectively. It concluded that pGFP had relative stability and Ah4332 GFP could be applied to bacterial ecology survey.