| 五种微绿球藻产油和产多不饱和脂肪酸的研究 |
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| 引用本文： | 李秀波,徐旭东,孔任秋.五种微绿球藻产油和产多不饱和脂肪酸的研究[J].水生生物学报,2010,34(5). |
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| 作者姓名： | 李秀波 徐旭东 孔任秋 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉430072;中国科学院研究生院,北京100049
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目，中国科学院太阳能行动计划 |
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| 摘 要： | 从5种微绿球藻中鉴别出4个高产油藻种和1个产油量很低的藻种.4种高产油微绿球藻在平台期油脂含量最高,占细胞干重的57%以上,其中三酰基甘油的含量占细胞干重的32.4%-45.2%.分析5种微绿球藻细胞的脂肪酸组成及4种高产油藻三酰基甘油中的脂肪酸组成,发现在高产油藻中,总的饱和脂肪酸和单不饱和脂肪酸的比例达到95%以上,多不饱和脂肪酸在5%以下,而在产油量很低的微绿球藻中多不饱和脂肪酸比例达45%以上.高产油微绿球藻三酰基甘油的多不饱和脂肪酸含量在4%以下,是生物柴油的优质原料,而产油量低的微绿球藻可用于提取C20:5脂肪酸(EPA).
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| 关 键 词： | 微绿球藻 三酰基甘油 脂肪酸 |
STUDIES ON THE PRODUCTION OF OIL AND POLYUNSATURATED FATTY ACIDS IN FIVE SPECIES OF NANNOCHLOROPSIS |
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| LI Xiu-Bo,XU Xu-Dong,KONG Ren-Qiu.STUDIES ON THE PRODUCTION OF OIL AND POLYUNSATURATED FATTY ACIDS IN FIVE SPECIES OF NANNOCHLOROPSIS[J].Acta Hydrobiologica Sinica,2010,34(5). |
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| Authors: | LI Xiu-Bo XU Xu-Dong KONG Ren-Qiu |
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| Institution: | LI Xiu-Bo1,2,XU Xu-Dong1 and KONG Ren-Qiu1 (1. Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,2. The Graduate University of Chinese Academy of Sciences,Beijing 100049) |
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| Abstract: | Nannochloropsis species are known for high contents of oil or polyunsaturated fatty acids (PUFA), such as EPA (C20:5). In Nannochloropsis species, oils contain essentially saturated and mono-unsaturated fatty acids, while polar lipids are rich in PUFA. In this study, total lipids, oils and fatty acids were analyzed in 5 Nannochloropsis species. Total lipids were extracted from cells using chloroform/methanol, and from total lipids, triacylglycerols were purified using thin layer chromatography. Of the five ... |
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| Keywords: | Nannochloropsis Triacylglycerols Fatty acids |
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