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鲤鱼管内皮细胞的分离培养及初步鉴定
引用本文:吴康,薛明强,吴海琴,李义,宋学宏,王裕兴.鲤鱼管内皮细胞的分离培养及初步鉴定[J].水生生物学报,2001,25(2):174-178.
作者姓名:吴康  薛明强  吴海琴  李义  宋学宏  王裕兴
作者单位:苏州大学水产学院,苏州 215151
基金项目:江苏省1995年农业重大攻关课题(编号:96369-2)
摘    要:鲤动脉球内灌注0.1%胶原酶消化,分离到大量内皮细胞及少量成纤细胞,28℃,在加入蛋白含量为:350μg/mL鲤下丘脑粗提液的1640+20%小牛血汪培养液中,细胞生长良好,7d后,接近单层。原代经三次0.5%柠檬酸胰酶消化逐步淘汰少量成纤维细胞,10d后,得纯内皮细胞,并顺利传至二代,第二代细胞经血凝ⅧR因子酶标检测发现,培养细胞存在血凝ⅧR因子相同抗原,具有内皮细胞的一般特征,从而得到初步鉴定。

关 键 词:  血管内皮细胞  培养  初步鉴定  分离培养
文章编号:1000-3207(2001)02-0174-05
修稿时间:1999年6月7日

SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO
WU Kang,XUE Ming-qiang,WU Hai-qin,LI Yi,SONG Xue-hong and WANG Yu-xing.SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO[J].Acta Hydrobiologica Sinica,2001,25(2):174-178.
Authors:WU Kang  XUE Ming-qiang  WU Hai-qin  LI Yi  SONG Xue-hong and WANG Yu-xing
Abstract:Many endothelial cells and few fibro-cells were separated fromthe artery ball Cyprinus carpio injected with 0.1% collagense(Ⅰ). At 28℃,in the 1640+10% calf bovine serum medium which was supplemented with 350μg/mL carp hypothulumus-derived growth substance, these cells grew well and the cell monolayers were obtained within 7-days period. By three times digestion of 0.5% trypsin-citric acid solution, the pure endothelial cells were gained after 10 days and they were subcultured to which. The second generation were proved to have ⅧR factor antigen by anti-ⅧR factor assay. These cells were identictied with general biological character of endothelial cells.
Keywords:Cyprinus carpio  Endothelial cells  Culture in vitro  Preliminary identification
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