帚状蜈蚣藻的修订研究——基于形态观察和rbcL序列分析

通过形态观察和分子分析相结合的方法，对青岛（模式标本产地）产的帚状蜈蚣藻（Grateloupia fastigiata Li et Ding）进行了重新鉴定，结果表明:①藻体为深紫红色，高9-26 cm，基部呈圆柱状，1-2回不规则羽状分枝，皮层厚度为85-160 μm，由8-11层细胞构成；助细胞生殖枝丛为典型的Grateloupia型，果胞枝生殖枝丛主枝由6个细胞组成，辅助细胞生殖枝丛主枝由5个细胞组成（6cpb-5auxb型），与亚洲蜈蚣藻（G.asiatica Kawaguchi et Wang）一致。②基于rbcL基因序列构建的系统树显示:本研究的10个帚状蜈蚣藻样本与青岛产的亚洲蜈蚣藻之间无碱基差异，形成独立的进化支，与大连石槽、石庙子产的亚洲蜈蚣藻的碱基差异均为1 bp（0.08%），与日本、韩国产的亚洲蜈蚣藻的碱基差异分别为2 bp（0.16%）、3 bp（0.24%），均属于种内差异，与Grateloupia clade I中蜈蚣藻属内其他种的碱基差异为18（1.47%）-76 bp（6.19%），均属于种间差异。通过形态观察和rbcL基因序列分析，认为帚状蜈蚣藻与亚洲蜈蚣藻为同一种，将帚状蜈蚣藻作为亚洲蜈蚣藻的异名。

RESEARCH ON THE REVISION OFGRATELOUPIA FASTIGIATA LI ET DING (RHODOPHYTA,HALYMENIACEAE) BASED ON MORPHOLOGICAL OBSERVATIONS ANDRBCL SEQUENCE ANALYSES

By combining morphological observation with molecular analysis,Grateloupia fastigiata Li et Ding was re-examined. The results showed that:① thallus was dark purplish red, 9—26 cm high, percurrent axes terete basally, di-chotomously branched once or twice, cortex sections were 85—160 m thick consisting of 8—11 layers of cells; the auxi-liary cell ampullae exhibited typicalGrateloupia-type, carpogonial branch ampullae consisted of six cells, auxiliary cell ampullae consisted of five cells (6cpb—5auxb type), identical withG. asiatica Kawaguchi et Wang.② According to the phylogenetic tree constructed by gene (rbcL) sequences, there was no sequence divergence between the ten samples ofG. fastigiata examined in this research andG. asiatica from Qingdao, which formed a single monophyletic subclade within the largeGrateloupia clade of Halymeniaceae, therbcL sequences differences between it andG. asiatica from Dalian was 1 bp (0.08%), therbcL sequences differences between it andG. asiatica from Japan and South Korea was 2 bp (0.16%) and 3 bp (0.24%), belonging to intraspecific difference, therbcL sequences differences between it and oth-er species within theGrateloupia clade I was 18 (1.47%)—76 bp (6.19%), belonging to interspecific difference. Through morphological observations and molecular analysis,G. fastigiata andG. asiatica proved to be the same spe-cies, withG. fastigiata as the synonym ofG. asiatica.