额尔齐斯河白斑狗鱼假尾复口吸虫的分子鉴定 |
| |
引用本文: | 番林古丽·热哈提,焦丽,岳城.额尔齐斯河白斑狗鱼假尾复口吸虫的分子鉴定[J].水生生物学报,2016,40(5):992-996. |
| |
作者姓名: | 番林古丽·热哈提 焦丽 岳城 |
| |
作者单位: | 新疆农业大学动物医学学院,乌鲁木齐,830052 |
| |
基金项目: | 国家自然科学基金项目(31360644)资助 Supported by the National Natural Science Foundation of China (31360644) |
| |
摘 要: | 利用分子生物学方法对采自额尔齐斯河(中国段)的白斑狗鱼(Esox lucius Linnaeus)晶状体内寄生的复口吸虫(Diplostomum)进行了种类鉴定,结果显示其种类为假尾复口吸虫(D.paracaudum),为中国新记录种。通过PCR扩增待鉴定种的部分18S-ITS1-5.8S序列,测序后与GenBank中假尾复口吸虫的序列进行比对,发现其相似度达到99.6%。将该序列与7种复口吸虫的ITS1序列进行比对后构建系统进化树,进化树以单殖吸虫Gyrodactylus parvae为外类群,结果显示:待鉴定种与假尾复口吸虫(D.paracaudum)聚为一支,其支持率达到了50%以上,支持它们为同一虫种。
|
关 键 词: | 额尔齐斯河 白斑狗鱼 假尾复口吸虫 分子鉴定 |
收稿时间: | 2016-02-19 |
MOLECULAR IDENTIFICATION OF DIPLOSTOMUM PARACAUDUM ON ESOX LUCIUSIN IRTYSH RIVER |
| |
Abstract: | The current study identified a species of Diplostomum from the lens of Esox lucius in Irtysh River (China section) using molecular biology techniques. These results showed that this species was Diplostomum paracaudum, a new record of this kind of species in China. The similarity of partial 18S-ITS1-5.8S sequence between this sample and another D. paracaudum from GenBank was 99.6%. A phylogenetic tree analysis based on the partial 18S-ITS1-5.8S of 7 species in the genus of Diplostomum identified an out group species Gyrodactylus parvae, and supported our sample was closely related to D. paracaudum with a more than 50% bootstrap. |
| |
Keywords: | Irtysh River Esox lucius Linnaeus Diplostomum paracaudum Molecular identification |
本文献已被 CNKI 万方数据 等数据库收录! |
| 点击此处可从《水生生物学报》浏览原始摘要信息 |
| 点击此处可从《水生生物学报》下载免费的PDF全文 |