玫瑰高原鳅肠道微生物多样性研究

研究旨在分析玫瑰高原鳅(Triplophysa rosa)肠道微生物的结构组成和多样性, 探索其肠道微生物的潜在功能。提取了5尾玫瑰高原鳅的肠道总DNA, 运用Illumina Miseq平台对肠道微生物16S rRNA的V3—V4区进行了测序, 统计样品肠道微生物的操作分类单元(Operational Taxonomic Units, OTUs)数量, 分析物种组成、丰度及Alpha多样性, 并预测肠道微生物的功能。结果显示, 玫瑰高原鳅的肠道微生物有19门、31纲、87目、 146科、253属、 320种, 451个OTUs。在门水平上, 优势菌群为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes); 在属水平上, 优势菌群为气单胞菌属(Aeromonas)、爱德华菌属(Edwardsiella)、邻单胞菌属(Plesiomonas)和希瓦氏菌属(Shewanella)。功能预测表明, 肠道微生物编码的大多数基因与新陈代谢相关, 其中“碳水化合物运输和代谢”和“氨基酸转运与代谢”功能类群的相对丰度较高。玫瑰高原鳅肠道内微生物组成复杂, 其功能分析有助于理解肠道微生物对玫瑰高原鳅摄食的影响, 为进一步研究玫瑰高原鳅的食性提供了理论基础。

ANALYSIS OF INTESTINAL MICROORGANISMS IN TRIPLOPHYSA ROSA(TELEOSTEI,CYPRINIFORMES)

Triplophysa rosa is a typical cave-dwelling fish distributed at Wulong County,Chongqing,China.It has degenerated eyes,albino skin,and highly developed sense organs,but its gut microbial function adapted to cave remains to be settled.The aims of this study were to analyze the structure and diversity of intestinal microorganisms in T.rosa and to explore the function of intestinal microorganisms.We extracted total intestinal DNA of 5 T.rosa,and then sequenced the V3—V4 region of 16S rRNA using Illumina Miseq sequencing technology.The number of operational taxonomic units(OTUs)of intestinal microorganisms was counted to analyze species composition,abundance,and Alpha diversity.The function of intestinal microorganisms was predicted by PICRUSt.A total of 451 OTUs of the high-quality sequences were obtained,belonging to 19 phyla,31 classes,87 orders,146 families,253 genera,and 320 species.At the phylum level,Proteobacteria,Actinobacteria,and Bacteroidetes were the dominant microorganisms.At the genus level,Aeromonas,Edwardsiella,Plesiomonas,and Shewanella were the main microorganisms.Functional prediction showed that most of the genes encoded by intestinal microorganisms are related to metabolism with the highest relationship with“amino acid transport and metabolism”and“carbohydrate transport and metabolism”.The composition of the microorganisms in the intestinal tract of T.rosa is complex,and functional analysis is helpful to understand the influence of intestinal microorganisms on its feeding,providing a theoretical basis for further study on fish diet.