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中华鳖4个Sox基因保守区的序列分析
引用本文:聂刘旺,单祥年,汪鸣,郭超文,鲁晓萱.中华鳖4个Sox基因保守区的序列分析[J].水生生物学报,2001,25(3):245-250.
作者姓名:聂刘旺  单祥年  汪鸣  郭超文  鲁晓萱
作者单位:1. 安徽师范大学生物系,芜湖,241000
2. 南京铁道医学院铁道部分子生物学开放实验室,南京,210097
基金项目:安徽省自然科学基金;江苏省自然科学基金(项目编号:97412002和97506112)
摘    要:采用PCR技术,扩增和克隆了中华鳖Sox基因(TSSox)。经DNA序列分析显示,Sox基因在系统进化上十分保守,其中TSSox4与鸟类LF4基因编码的氨基酸序列完全相同、与人类SOX4和Sox4编码的序列仅一个氨基酸的差异;TSSox5与鸟类的LF5基因的编码也仅一个氨基酸发生了改变;TSSox2与海龟的TSox2相似性最高。4条TSSox序列中,TSSox与人SRY基因序列相似性最高,达75%;序列上的相似性可能暗示了它们在功能上的保守性。

关 键 词:中华鳖  Sox基因  序列分析  PCR  保守区  性别决定
文章编号:1000-3207(2001)03-0245-06
修稿时间:2000年4月7日

THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS
NIE Liu-wang ,SHAN Xiang-nian ,WANG Ming ,GUO Chao-wen and LU Xiao-xuan.THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS[J].Acta Hydrobiologica Sinica,2001,25(3):245-250.
Authors:NIE Liu-wang  SHAN Xiang-nian  WANG Ming  GUO Chao-wen and LU Xiao-xuan
Institution:NIE Liu-wang 1,SHAN Xiang-nian 2,WANG Ming 1,GUO Chao-wen 1 and LU Xiao-xuan 2
Abstract:The Sox genes of Trionyx sinensis were amplified and cloned using PCR. The four different fragments of Sox gene HMG-box DNA were selected and sequenced. The results indicated that the Sox gene is conservative in phylogeny, in which the amino acid sequence encode by TSSox4 (The Sox of T. Sinensis ) is identical to those encode by LF4 (bird) or differ from SOX4(human) and Sox4(mouse) only on one amino acid; The amino acid sequences encode by TSSox5 have an amino acid difference from those encode by LF5(bird); TSSox1 has about 75% identical to SRY HMG-box at the nucleotide level, and is most resemblance among four TSSox. Phylogenetic analysis of available 32 Sox sequences including SRY/Sry sequence suggests that there wolud be a high degree of divergence between any possible immediate common ancestor of the T. Sinensis Sox sequence and the SRY(Sry) sequences.
Keywords:Trionyx sinensis  Sox gene  Sequence analysis  PCR
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