基于第二代测序技术兰州鲇线粒体基因组全序列测定与分析

研究采用高通量第二代测序技术，构建获得兰州鲇（Silurus lanzhouensis）线粒体基因组全序列，并对全序列特征和结构进行了分析。研究结果表明，兰州鲇线粒体基因组全序列长度为16523 bp，碱基组成具有高A+T低G+C含量的偏向性，具有脊椎动物典型的结构组成。13个PCG基因中存在2种启动子（ATG、GTG）、3种终止子（TAG、TAA和T或TA）。除tRNA-Ser（AGN）基因二级结构中DHU臂缺失，其余21个tRNA基因可折叠成典型三叶草结构。12S rRNA二级结构由45个茎环结构组成4个结构域，16S rRNA由54个茎环结构组成6个结构域。含有关键序列标签的控制区（CR）可分为3个不同的结构域:终止序列区（TAS1、TAS2）、中央保守区（CSB-F、CSB-E和CSB-D）和保守序列区（CSB1、CSB2和CSB3）。非编码区含有一段保守的控制轻链复制起始的序列区（O_L）。基于线粒体基因组全序列和通用标签COX1基因标记可区分兰州鲇同其他鲇形目鱼类种质进化关系。

SEQUENCING AND ANALYSIS OF THE COMPLETE MITOCHONDRIAL GENOME OF SILURUS LANZHOUENSIS BASED ON NEXT GENERATION SEQUENCING TECHNOLOGIES

In this study, the high-throughput next-generation sequencing technology was used to build Lanzhou catfish (Silurus lanzhouensis) mitochondrial genome sequence, and the complete sequence and characteristics for mitochond-rial genome has been analyzed. The main results were demonstrated in detail. The circular genome was 16523 bp in length with the typical mtDNA components, and the base composition showed a tendency of high A+T and low G+C. Two promoters (ATG, GTG) and three terminators (TAG, TAA and T or TA) were found in all 13 PCGs. Except tRNA-Ser(AGN) gene showed the shortage of DHU, the other 21 genes of tRNA could be folded in clover shape. The 45 stem-loop structures and 4 structural domains were identified in secondary structure of 12S rRNA, and 54 stem-loop struc-tures and 6 structural domains were identified in 16S rRNA. Three different structural domains of termination se-quence region (TAS1, TAS2), central conservative region (CSB-F, CSB-E and CSB-D) and conservative region (CSB1, CSB2 and CSB3) were divided in the controlled region that remains key sequence label. The non-code region has a conserva-tive sequence to control light chain starting copy. The complete sequence of mitochondrial genome and general COX1 label could be used for the study of evolutionary relationship between S. lanzhouensis and other Siluriformes species.