群体和单细胞微囊藻对短期温度变化的生理响应

为了探索短期温度变化对群体微囊藻和单细胞微囊藻的影响, 在室内受控模拟条件下研究了在10℃、25℃和35℃三个温度梯度下, 群体和单细胞微囊藻对短期温度变化的生理响应。研究表明: 与对照组25℃相比, 在10℃培养下, 微囊藻叶绿素浓度显著降低, SOD活性和死亡率均显著增加。与群体微囊藻相比, 在10℃下单细胞微囊藻叶绿素浓度显著下降, F_v/F_m下降, SOD活性显著增加。在35℃培养下, 单细胞微囊藻叶绿素浓度上升, 死亡率和SOD活性增加, 而群体微囊藻则呈现出叶绿素浓度和死亡率降低, CAT活性增加。结果表明短期的温度变化影响了群体和单细胞微囊藻生理机制, 与单细胞微囊藻相比, 群体更能适应短期的温度胁迫, 导致其更具优势。

THE PHYSIOLOGICAL RESPONSE OF COLONIAL AND SINGLE-CELLED FORM OF MICROCYSTIS TO SHORT-TERM TEMPERATURE CHANGES

To investigate the effect of short-term temperature changes on the colonial and single-cell Microcysis forms, physiological responses were determined after it was cultured at 10℃, 25℃, and 35℃. The results demonstrated that a decline in chlorophyll-a and an increase in superoxide dismutase (SOD) and mortality rates were found when Micro-cystis was cultured at 10℃ when compared to those cultured at 25℃. Significant decreases in chlorophyll-a and Fv/Fm were observed in the Microcystis single-cell form in contrast to the colonial form, while SOD activity significantly in-creased when Microcystis was inoculated at 10℃. After culturing at 35℃, chlorophyll-a, mortality rate, and SOD levels significantly increased in single-cell Microcystis, however, a significant decrease in chlorophyll-a and mortality rates and a significant increase in catalase activity was found in colonial Microcystis. The results suggested that short-term temperature stress could affect physiological mechanisms in colonial and single-cell Microcystis. However, colonial Microcystis showed more advantages in adapting to short-term temperature stress than the single-cell form.