饲料中添加磷虾水解物对大菱鲆幼鱼生长性能、体组成及相关酶活性的影响

选取初始体重为(9.46±0.01) g的大菱鲆(Scophthalmus maximus L.)为研究对象, 以30%鱼粉组为对照, 分别添加5%磷虾水解物(LKH)和10%磷虾水解物(HKH)以替代鱼粉蛋白, 配制3组等氮等脂的饲料, 在室内流水养殖系统进行为期10周的养殖实验, 旨在探究饲料中添加磷虾水解物对大菱鲆幼鱼生长性能、体组成及相关酶活性的影响。结果显示, HKH组大菱鲆幼鱼的特定生长率(SGR)、饲料效率(FE)、蛋白质效率比(PER)、蛋白质沉积率(PPV)均显著高于对照组(P<0.05), 但摄食率(FI)显著低于对照组(P<0.05); LKH、HKH组大菱鲆幼鱼肌肉总氨基酸含量和必需氨基酸含量显著高于对照组(P<0.05); LKH、HKH组肝脏谷草转氨酶(GOT)活性显著高于对照组(P<0.05), 而血清GOT活性与对照组无显著性差异(P>0.05); HKH组肝脏谷丙转氨酶(GPT)活性显著高于对照组和LKH组(P<0.05), 但血清GPT活性显著低于LKH组和对照组(P<0.05); HKH组肠道淀粉酶活性显著高于对照组(P<0.05), 同时随着磷虾水解物添加量的升高, 肠道及幽门盲囊胰蛋白酶活性先降低后升高, 且在HKH组显著高于对照组(P<0.05)。综上结果表明, 在饲料中添加10%磷虾水解物能够提高大菱鲆幼鱼的生长性能, 促进其氨基酸代谢和消化吸收。

EFFECTS OF DIETARY KRILL HYDROLYSATES ON GROWTH PERFORMANCE,BODY COMPOSITION AND RELATED ENZYME ACTIVITIES OF JUVENILE TURBOT (SCOPHTHALMUS MAXIMUS L.)

The current study investigated the effects of dietary krill hydrolysates on growth performance, body compo-sition and related enzyme activities of juvenile turbot (Scophthalmus maximus L.) with a 10-week feeding trial using in-door flowing-through seawater system. Three isonitrogenous and isolipidic diets were formulated to contain 0 (Control), 5% (LKH), 10% (HKH) krill hydrolysates for substitution of fish meal protein in basal diet that includes 30%fish meal, respectively. The results showed that the specific growth rate (SGR), feed efficiency rate (FE), protein effi-ciency ratio (PER) and protein productive value (PPV) in HKH group were significantly higher than those in the con-trol group, but the feed intake (FI) was significantly lower than that of the control group. HKH supplementation had significantly higher whole-body moisture, whole-body crude protein contents and whole-body lipid contents than those in the control group (P<0.05). Both LKH and HKH groups significantly enhanced the muscle total amino acids con-tents and essential amino acids content compared with control group (P<0.05). Compared with the control group, both LKH and HKH significantly induced glutamic oxalacetic transaminase (GOT) activities in liver but not GOT activities in serum. HKH group showed the highest glutamic-pyruvic transaminase (GPT) activities in liver and the lowest GPT activities in serum. HKH group significantly increased amylase activity in intestines. The increasing amount of krill hy-drolysates supplied in diet first decreased and then increased trypsin activities in pyloric caecum and intestines, and HKH group had significantly higher trypsin activities in pyloric caecum and intestines than those in the control group. In conclusion, 10% krill hydrolysates supplementation in diets could promote growth performance, amino acid meta-bolism as well as digestion and absorption of juvenile turbot.