饥饿再投喂对大鳞副泥鳅生长性能、肝脏抗氧化能力和肠道消化酶的影响

为探究周期性饥饿再投喂对大鳞副泥鳅(Paramisgurnus dabryanus)生长性能、抗氧化能力和肠道消化酶活性的影响, 实验将初始重一致的大鳞副泥鳅随机分为4组, 每组3个重复, 饲养于12个水箱中, 每箱20尾。采用周期性饥饿2d再投喂4d(S2F4)、周期性饥饿2d再投喂6d(S2F6)、周期性饥饿2d再投喂8d(S2F8)和持续投喂(对照组)4种投喂模式, 投喂30d, 并于第0、第15和第30天收集样本进行检测。结果表明: (1)不同处理对末体长和特定生长率无显著影响(P>0.05), S2F8处理组末体重和增重率显著高于对照组(P<0.05)。(2)周期性饥饿再投喂对肥满度、脏体比和肝体比无显著影响(P>0.05)。(3)随饥饿再投喂处理时间增长, S2F6和S2F8组肝脏SOD、CAT和GSH-PX活性显著升高; 在第15天, S2F8组SOD活性显著高于对照组(P<0.05), S2F6和S2F8组肝脏CAT活性显著高于对照组(P<0.05), S2F6和S2F8组肝脏GSH-PX活性均显著高于对照组(P<0.05)。在第30天, S2F6和S2F8组SOD活性显著高于对照组(P<0.05), S2F6组CAT活性均显著高于对照组(P<0.05), S2F6和S2F8组中GSH-PX活性显著高于对照组(P<0.05)。(4)对肠道消化酶研究发现, 投喂时间对肠道蛋白酶、淀粉酶和脂肪酶活性无显著影响。在第30天时, S2F6和S2F8组肠道脂肪酶显著低于对照组(P<0.05)。综上所述, 周期性饥饿再投喂可激发大鳞副泥鳅补偿生长, 引起肝脏抗氧化酶活性增加, 肠道消化酶活性降低。其中S2F8组补偿生长最显著, 且肠道消化酶活性变化程度较小。因此, 为保证饲养效果, 推荐使用S2F8投喂模式。

PERIODICAL STARVATION-REFEEDING ON GROWTH PERFORMANCE,HEPATIC ANTIOXIDANT INDICES,INTESTINAL DIGESTIVE ENZYME ACTIVITIES OF PARAMISGURNUS DABRYANUS

To explore the effects of periodical starvation-refeeding on growth performance, antioxidant indices, digestive enzyme activities of Paramisgurnus dabryanus, the P. dabryanus were divided into 4 groups, including starvation 2d and refeeding 4d (S2F4 group), starvation 2d and refeeding 6d (S2F6 group), starvation 2d and refeeding 8d (S2F8 group) and continuous feeding (control group) for a 30-days trial. The results showed that (1) the body length and specific growth rate were similar among groups (P>0.05), and the body weight and weight gain ratio of S2F8 were significantly higher than those of the control group (P<0.05). (2) The condition factor, visceral index and hepatosomatic index showed no differences among groups. (3) At 15d, the activity of SOD in S2F8 and CAT in S2F6 and S2F8 increased significantly compared with those of the control group (P<0.05), the activity of GSH-PX in S2F6 and S2F8 was higher than that of the control group (P<0.05). At 30d, the activity of SOD in S2F6 and S2F8 and CAT in S2F6 were higher than those of the control group (P<0.05), the activity of GSH-PX in S2F6 and S2F8 was higher than that of the control group (P<0.05). (4) The activity of protease, amylase and lipase showed no difference between each time (P>0.05). At 30d, the activities of lipase in S2F6 and S2F8 were lower than those of the control group (P<0.05). These results indicated compensatory growth in the periodical starvation-refeeding groups with the increase of antioxidant enzyme activity and the decrease of digestive enzyme activity, suggesting that the feeding mode of S2F8 can be used in P. dabryanus culture.