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细胞松弛素对嗜热四膜虫皮层细胞骨架蛋白的影响
引用本文:庞延斌,李书强.细胞松弛素对嗜热四膜虫皮层细胞骨架蛋白的影响[J].水生生物学报,2002,26(5):501-504.
作者姓名:庞延斌  李书强
作者单位:华东师范大学生物系,上海,200062
基金项目:ThisworkwassupportbygrantsfromtheNSFC (No.3 9870 0 94)
摘    要:测定了细胞松弛素B对嗜热四膜虫RF1株和BF5在生殖接合期的皮层骨架蛋白,尤其是在接合子接合膜形成和原核交换阶段影响甚大。作者发现VB处理后,与对照组相比较,皮层骨架蛋白146KD消失,27KD,43KD,47KD和174KD含量下降,32KD,41KD,51KD和54KD保持不变,结果显示,松弛素B对微纤毛蛋白27KD、43KD、47KD、146KD和174KD有影响。

关 键 词:细胞松弛素  嗜热四膜虫  皮层细胞骨架蛋白

STUDIES ON THE EFFECT OF CYTOCHALASIN B ON MAJOR CO RTICAL CYTOSKELETAL PROTEINS OF TETRAHYMENA THERMOPHILA DURING CONJUGATION
PANG Yan,bin and LI Shu,qiang.STUDIES ON THE EFFECT OF CYTOCHALASIN B ON MAJOR CO RTICAL CYTOSKELETAL PROTEINS OF TETRAHYMENA THERMOPHILA DURING CONJUGATION[J].Acta Hydrobiologica Sinica,2002,26(5):501-504.
Authors:PANG Yan  bin and LI Shu  qiang
Abstract:The effect of cytochalasin B on cortical cytoskeletal proteins of the complementary mating types of Tetrahymena thermophila BF 1 and BF 5 during their conjugation was carried out, especially during the initial stage of fusion membrane formation stage and the stage of pronuclei exchange. We found that after the treatment of CB, 146KD cortical cytoskeletal protein disappeared, while it existed in the control group, and the percent of 27KD?43KD?47KD and 174KD declined, but the percent of 32KD?41KD?51KD and 54KD kept almost unchanged. Thus we concluded that 27KD?43KD?47KD?146KD and 174KD may be the microfilament proteins.
Keywords:Cytochalain B  Tetrahymena thermophila  Conjugation cortical cytoskeletal protein
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