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| 与“全红”瓯江彩鲤体色相关的SRAP及SCAR分子标记 | |
| 引用本文： | 吕耀平,胡则辉,王成辉,项松平,苏小平,姚子亮,王凯伟.与“全红”瓯江彩鲤体色相关的SRAP及SCAR分子标记[J].水生生物学报,2011,35(1):45-50. |
| 作者姓名： | 吕耀平 胡则辉 王成辉 项松平 苏小平 姚子亮 王凯伟 |
| 作者单位： | 1. 丽水学院化学与生命科学学院,丽水,323000;浙江大学动物科学学院,杭州,310029 2. 浙江省海洋水产研究所,舟山,316100 3. 上海海洋大学农业部水产种质资源与利用重点开放实验室,上海,200090 4. 浙江省龙泉市瓯江彩鲤原种场,龙泉,323700 5. 浙江省丽水市莲都区水产技术推广站,丽水,323000 6. 浙江省丽水市水产技术推广站,丽水,323000 7. 浙江省丽水市渔政站,丽水,323000 |
| 基金项目： | 国家自然科学基金(30700622); 浙江省自然基金项目(Y307445)资助 |
| 摘 要： | 利用相关序列扩增多态性(Sequence Related Amplified Polymorphism,SRAP)技术分析"全红"和"粉玉"瓯江彩鲤,筛选与瓯江彩鲤体色相关的分子遗传标记。从88个SRAP引物组合筛选出的12个引物组合共获得扩增条带104个,并筛选出1个SRAP特异扩增带,即"全红"瓯江彩鲤家系SR2,7173 bp带。该条SRAP特异扩增条带经回收、克隆和测序,并将测序结果进行BLAST分析,发现该片段在GenBank中与斑马鱼的POl多蛋白基因和尿红素基因有较高的同源性。根据序列信息分别设计了4对正、反向引物(22—26 bp)。用4对引物分别在"全红"瓯江彩鲤F2和"粉玉"瓯江彩鲤F2群体中进行PCR扩增,仅发现SC-3(154 bp)能够在"全红"瓯江彩鲤群体中特异扩增,而且在"粉玉"瓯江彩鲤F2群体中未出现此扩增带。采用大样本对该SC-3标记进行验证,结果发现,在"全红"瓯江彩鲤群体中呈现阳性,而在"粉玉"瓯江彩鲤群体中为阴性,可以区分这两种群体。因此SC-3标记可以作为"全红"瓯江彩鲤群体一个重要的分子遗传特征指标,为进一步进行分子标记辅助育种奠定了基础。 |
| 关 键 词： | “全红” “粉玉” 瓯江彩鲤 SRAP标记 SCAR标记 |
SRAP AND SCAR MOLECULAR MARKER RELATED TO BODY COLOR OF "WHOLE RED" PATTERNS OF CYPRINUS CARPIO VAR.COLOR |
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| L Yao-Ping,HU Ze-Hui,WANG Cheng-Hui,XIANG Song-Ping,SU Xiao-Ping,YAO Zi-Liang,WANG Kai-Wei.SRAP AND SCAR MOLECULAR MARKER RELATED TO BODY COLOR OF "WHOLE RED" PATTERNS OF CYPRINUS CARPIO VAR.COLOR[J].Acta Hydrobiologica Sinica,2011,35(1):45-50. | |
| Authors: | L Yao-Ping HU Ze-Hui WANG Cheng-Hui XIANG Song-Ping SU Xiao-Ping YAO Zi-Liang WANG Kai-Wei |
| Institution: | LU Yao-Ping1,2,HU Ze-Hui3,WANG Cheng-Hui4,XIANG Song-Ping5,SU Xiao-Ping7,YAO Zi-Liang6 and WANG Kai-Wei8(1.College of Chemistry and Life Sciences,Lishui University,Lishui 323000,2.College of Animal Science,Zhejiang University,Hangzhou 310029,3.Marine Fisheries Research Institute of Zhejiang Province,Zhoushan 316100,4.Key Laboratory of Aquatic Genetic Resources and Utilization,Ministry of Agriculture,Shanghai Ocean University,Shanghai 200090,5.Provincial Farm of Oujiang Color Common Carp,Longquan 323700,6... |
| Abstract: | Oujiang color common carp distributes in Zhejiang Province in southeast part of China,such as Lishui City,Longquan City,Qingtian country and Oujiang river basin.It has several color patterns,including whole red,red with scattered black spots,whole white,white with scattered black spots.Nowadays,several scientific researchers have car-ried out some works relevant to genetic diversity,genetic relationship in Cyprinus carpio var.color.However,we do not find any other studies on color-related molecular markers ... |
| Keywords: | Whole red patterns Whole White patterns Cyprinus carpio var color SRAP marker SCAR marker |
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