| 转兔防御素基因小球藻异养培养的培养基优化研究 |
| |
| 引用本文： | 韩兴梅,李元广,魏晓东,孙勇如,王义琴.转兔防御素基因小球藻异养培养的培养基优化研究[J].水生生物学报,2006,12(5):547-552. |
| |
| 作者姓名： | 韩兴梅 李元广 魏晓东 孙勇如 王义琴 |
| |
| 作者单位： | 1. 生物反应器工程国家重点实验室,华东理工大学海洋生化工程研究所,上海,200237
2. 中国科学院遗传与发育生物学研究所,北京,100101 |
| |
| 基金项目： | 国家863计划海洋生物技术主题资助项目(2002AA629110) |
| |
| 摘 要： | 在氮源种类筛选的基础上,采用Plackett-Burman实验设计并结合单因素实验对转兔防御素基因小球藻异养培养的培养基进行了优化。实验结果表明,采用优化后的Knop异养培养基在250mL摇瓶中进行异养培养,藻细胞密度从优化前的1.5g/L提高到优化后的5.11g/L;摇瓶和5L生物反应器异养培养表明,Knop异养培养基中藻细胞浓度分别为优化前培养基的3.39倍和3.17倍,而兔防御素(NP-1)表达能力基本不变。
|
| 关 键 词： | 转基因小球藻 兔防御素 异养培养 培养基优化 |
| 文章编号： | 1000-3207(2006)05-0547-06 |
| 修稿时间： | 2005年8月3日 |
Optimization of heterotrophic medium for culturing transgenic Chlorella with rabbit defensin gene |
| |
| HAN Xing-Mei,LI Yuan-Guang,WEI Xiao-Dong,SUN Yong-Ru,WANG Yi-Qin.Optimization of heterotrophic medium for culturing transgenic Chlorella with rabbit defensin gene[J].Acta Hydrobiologica Sinica,2006,12(5):547-552. |
| |
| Authors: | HAN Xing-Mei LI Yuan-Guang WEI Xiao-Dong SUN Yong-Ru WANG Yi-Qin |
| |
| Abstract: | Based on the experiment of screening nitrogen sources,mixotrophic Knop medium was optimized by using of Plackett-Burman experimental design and the one-at-a-time strategy and the cell density of transgenic Chlorella was improved from 1.12g/L to 5.11g/L in 250mL flasks.Using the optimized medium,the cell density of the transgenic Chlorella in 250 mL flasks and 5L bioreactor were 3.39 and 3.17 fold respectively what can be obtained in the mixotrophic Knop medium while the expression capabilities of NP-1 were kept unchanged. |
| |
| Keywords: | Transgenic Chlorella Rabbit defensin Cell density Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《水生生物学报》浏览原始摘要信息 |
| 点击此处可从《水生生物学报》下载免费的PDF全文 |
|
