光合细菌H3菌株色素分析

H3菌株系由盐田微生物层中分离获得的光合细菌株。具有丰富的天然色素。经活细胞色素光谱吸收峰值测定，色素经有机溶剂提取、硅胶薄板层析、SDS－PAGE电泳等，结果表明H3菌株的主要色素包括细菌叶绿素a、细菌脱镁叶绿素（Bacteriophaeophytin）和三种类胡萝卜素。总胡萝卜素含量占细胞于重的0．6％，胡萝卜素蛋白复合体的分子量约11，000.培养条件的差异对色素形成及相对含量有不同程度的影响。

ANALYSIS OF PIGMENTS IN PHOTOSYNTHETIC BACTERIUM STRAIN H_3

Photosynthehc bacterium strain H3 was isolated from microbial mat of evaporation pond in the saltern of Hainan Island. The absorphon spectrum of living cells showed characteristics peaks of bacteriochlorophyll a and the higher peak of carotenoids.Thin-layer chromatography revealed 5 major photosynthetic pigments including 3 carotenoids with absorption maxima at 485 nm and 516nm, 481nm and 510nm, 486tim and 521nm, respectively in acetone. The total carotenoid content is about 0.6% of dry cell weight An easily solubilized carotenoid - contaning protein has been purified, and SDS-PAGE indicated a single polypeptide of MW 11,000 in the orange protein. A bacceriopnaeophytin is also found. Culture conditions such as light and temperature effect on pigment composihons were examined in photosynthetic bacteria.