草鱼两个细胞因子信号抑制因子3基因序列及表达分析

细胞因子信号抑制因子3 (SOCS3)是一类调节免疫反应的蛋白, 为研究其在草鱼(Ctenopharyngodon idella)中的功能, 文章克隆了草鱼SOCS3b基因, 分析了SOCS3s基因在成鱼组织中的表达情况。序列分析结果显示, 草鱼SOCS3b基因全长2126 bp, 编码216个氨基酸。qRT-PCR结果显示, 草鱼SOCS3a和SOCS3b在成鱼11个组织中均有表达, 但表达略有差异。注射嗜水气单胞菌(Aerononas hydrophila)后, 草鱼SOCS3a和SOCS3b在肝、脾、肠、肾中的表达均有明显上升。以上结果表明SOCS3s基因在草鱼的组织生长调控中发挥着重要的作用, 且SOCS3s可以调节细菌诱导的免疫应答。研究将为后续草鱼SOCS3s基因的功能研究提供参考依据。

TISSUE DISTRIBUTION OF TWO SUPPRESSOR OF CYTOKINE SIGNALING 3 (SOCS3) GENES AND THEIR ROLE IN BACTERIA-INDUCED INNATE IMMUNE RESPONSE IN GRASS CARP (CTENOPHARYNGODON IDELLA)

The suppressor of cytokine signaling 3 (SOCS3) regulates the immune response. Here, we cloned the SOCS3b gene and investigated their role and distribution of SOCS3s in grass carp (Ctenopharyngodon idella). Grass carp SOCS3b gene was 2126 bp in length, encoding 216 aa peptides. In adult fish, both orthologs were expressed in 11 tissues with slightly different among tissues. After Aerononas hydrophila injection, both SOCS3a and SOCS3b were significantly up-regulated in the liver, spleen, intestine and kidney tissues. These results suggested that SOCS3s may play important roles in tissue growth of grass carp and the modulation of bacteria-induced innate immune response. This study provides relevant reference for the follow-up study of the function of SOCS3s genes in grass carp.