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低等鲤科鱼类RAPD分析及系统发育研究
引用本文:何舜平,王伟,陈宜瑜.低等鲤科鱼类RAPD分析及系统发育研究[J].水生生物学报,2000,24(2):101-106.
作者姓名:何舜平  王伟  陈宜瑜
作者单位:中国科学院水生生物研究所,武汉 430072
基金项目:国家自然科学基金课题:低等鲤科鱼类分子进化过程及系统发育研究,批准号:39500016.
摘    要:通过对鲤科鱼类30个个体所代表的18个种的随机扩增,作者得到了大量有系统发育信息的DNA多态片段。通过Rapdplot程序将DNA的多态片段转换成遗传距离(d=1-S,S=2NxNy/Nx+Ny)。该遗传距离的矩阵经PHYLIP软件包中的Neighbor(option=NJ)程序处理后,生成了低等鲤科鱼类代表属种的分支系统图。从该系统图中以看出:RAPD分析方法在鲤科鱼类的系统发育研究中有一定的局

关 键 词:鲤科  系统发育  分子进化  RAPD分析
文章编号:1000-3207(2000)02-0101-06
修稿时间:1999年1月20日

THE RAPD ANALYSIS AND THE PHYLOGENETIC CONCERNING FOR PRIMITIVE CYPRINIDAE
HE Shun-ping, WANG Wei and CHEN Yi-yu.THE RAPD ANALYSIS AND THE PHYLOGENETIC CONCERNING FOR PRIMITIVE CYPRINIDAE[J].Acta Hydrobiologica Sinica,2000,24(2):101-106.
Authors:HE Shun-ping  WANG Wei and CHEN Yi-yu
Abstract:Many informative fragments of the RAPD were obtained from 30 individuals that representing 20 species of the primitive cyprinid fishes by the method of random amplifying with 20 primers. We converted the DNA polymorphic fragment into genetic distance by the program Rapdplot (d=1-S, S=2NxNy/Nx Ny). The prograxns Neighbor in software package PHYLIP was used to calculated the matrix of genetic distance resulted which in one cladogram of 20 species. It is indicated that the application of the RAPD analysis for Cyprinidae may have some limitations. For the inter-genera analysis in same subfamily, the result is reliable. The result of the RAPD analysis indicates that: the subfamily Danioinae is not a monophyletic group.Among them, the Zacco group and Aphyocypris group form monophyly respectively. And in this subfamily, the two groups do not have a direct relationship.
Keywords:Cyprinidae  Phylogenetics  Molecular evolution  RAPD analysis
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