| 鲸类微卫星引物对长江江豚的适用性研究 |
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| 引用本文： | 夏军红,郑劲松,王丁.鲸类微卫星引物对长江江豚的适用性研究[J].水生生物学报,2004,28(6):640-646. |
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| 作者姓名： | 夏军红 郑劲松 王丁 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072;中国科学院研究生院,北京,100039
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 国家自然科学基金 (3 0 170 14 2 )，中国科学院重要创新方向项目(KSCX2 SW 118)，中国科学院创新领域前沿项目(2 2 0 10 3 )资助 |
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| 摘 要： | 微卫星在长江江豚 (Neophocaenaphocaenoidesasiaeorientalis)中的应用研究还未见报道。本研究采用已发表的来自 6个鲸种的 2 3对微卫星引物对一个长江江豚群体DNA样本进行了微卫星扩增。结果表明其中有 7对引物在此群体中的扩增产物是稳定且多态的 ,序列分析结果表明这 7对引物的扩增产物都具有AC或GT两碱基重复单元 ,从而证明了扩增的有效性。研究结果表明用从其他鲸类分离出的微卫星引物可以快速筛选到适用于长江江豚指纹分析的引物
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| 关 键 词： | 长江江豚 微卫星 筛选 |
| 文章编号： | 1000-3207(2004)06-0640-07 |
| 修稿时间： | 2003年10月13 |
APPLICABILITY OF CETACEAN MICROSATELLITE PRIMERS IN THE YANGTZE FINLESS PORPOISE |
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| XIA Jun-Hong ,ZHENG Jin-Song , and WANG Ding.APPLICABILITY OF CETACEAN MICROSATELLITE PRIMERS IN THE YANGTZE FINLESS PORPOISE[J].Acta Hydrobiologica Sinica,2004,28(6):640-646. |
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| Authors: | XIA Jun-Hong ZHENG Jin-Song and WANG Ding |
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| Institution: | XIA Jun-Hong 1,2,ZHENG Jin-Song 1,2 and WANG Ding 1 |
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| Abstract: | Application of microsatellites in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) has not been reported.In this study amplification of microsatellites were performed in DNA samples of the Yangtze finless porpoise population by PCR using twenty-three published microsatellite primers from Phocaena phocoena,Eubalaena glacialis,Physeter catodon,Megaptera novaeangliae,Tursiops truncates,Globicephala melas. The results indicated seven primer pairs produced stable and polymorphic PCR products in the population.Further sequencing analysis showed that the amplified product sequences of the seven primer pairs all own AC or GT di-nucleotide repeats,which confirmed that the amplification was valid.The results suggest that applicable primers in fingerprinting analysis of the Yangtze finless porpoise could be obtained faster by screening of microsatellite primers isolated from other cetacean species. |
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| Keywords: | The Yangtze finless porpoise Microsatellite Screening |
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