| 水体pH胁迫对异育银鲫皮质醇激素和非特异性免疫的影响 |
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| 引用本文： | 李文祥,谢骏,宋锐,邹红,吴山功,王桂堂.水体pH胁迫对异育银鲫皮质醇激素和非特异性免疫的影响[J].水生生物学报,2011,35(2):256-261. |
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| 作者姓名： | 李文祥 谢骏 宋锐 邹红 吴山功 王桂堂 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072
2. 中国水产科学院淡水渔业研究中心,无锡,214081 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助(NYCYTX-49-15); 国家重点基础研究发展计划(973)项目(2009CB118705)资助 |
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| 摘 要： | 实验比较了不同pH胁迫对异育银鲫(Carassius auratus gibelio)皮质醇激素和非特异性免疫的影响。3个实验组水质的pH分别为6.0、8.5、9.5,对照组是pH为7.4的曝气自来水。分别在实验的第0、14和30天采血,血浆用于测定皮质醇含量和溶菌酶活性;分离的白细胞,采用流式细胞术测定吞噬活性和呼吸暴发。在实验的第4天多取1次血样,用于皮质醇含量测定。在实验的第31天,测量鱼的体重,并用柱状黄杆菌(Flavobacterium columnare)浸泡攻毒,7d后统计死亡情况。结果显示:在pH 6.0的胁迫下,白细胞的吞噬活性在第14、30天显著降低,而呼吸暴发和皮质醇含量没有显著变化,溶菌酶活性和生长与对照组没有显著差异,用黄杆菌攻毒也没有出现死亡。而在pH 8.5和9.5的胁迫下,皮质醇含量在第14、30天显著升高,白细胞的吞噬活性、呼吸暴发显著降低;在pH 9.5胁迫下的溶菌酶活性显著降低,用柱状黄杆菌攻毒两组都出现死亡。实验表明pH为8.5和9.5的胁迫严重影响了异育银鲫的非特异性免疫功能,流式细胞术能较客观测定白细胞的吞噬活性和呼吸暴发,可用于评价胁迫对鱼类健康的影响。
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| 关 键 词： | pH胁迫 异育银鲫 皮质醇 溶菌酶活性 吞噬活性 呼吸暴发 流式细胞术 |
EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF CARASSIUS AURATUS GIBELIO |
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| LI Wen-Xiang,XIE Jun,SONG Rui,ZOU Hong,WU Shan-Gong,WANG Gui-Tang.EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF CARASSIUS AURATUS GIBELIO[J].Acta Hydrobiologica Sinica,2011,35(2):256-261. |
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| Authors: | LI Wen-Xiang XIE Jun SONG Rui ZOU Hong WU Shan-Gong WANG Gui-Tang |
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| Institution: | LI Wen-Xiang1,XIE Jun2,SONG Rui1,ZOU Hong1,WU Shan-Gong1 and WANG Gui-Tang1(1.Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,2.Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081) |
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| Abstract: | Effects of pH stress on cortisol and non-specific immunity of Carassius auratus gibelio were investigated.Acidity and Alkalinity of the aquaculture water were maintained at pH 6.0,7.4,8.5 and 9.5,where pH 7.4 was used as control.pH value of the aquaculture water was adjusted by HCl(1 N) or NaOH(1 N).Fish blood was sampled at 0,14 and 30 days during the experiment.Leucocytes isolated by 55% percoll were used for determination of phagocytosis and respiratory burst activity by flow cytometry,and the plasma was... |
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| Keywords: | pH stress Carassius auratus gibelio Cortisol Lysozyme activity Phagocytosis Respiratory burst activity Flow cytometry |
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