| CpG寡脱氧核苷酸触发中华绒螯蟹酚氧化酶原系统信号传导途径的研究 |
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| 引用本文： | 李红霞,俞菊华,李义,徐跑.CpG寡脱氧核苷酸触发中华绒螯蟹酚氧化酶原系统信号传导途径的研究[J].水生生物学报,2011,35(1):122-131. |
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| 作者姓名： | 李红霞 俞菊华 李义 徐跑 |
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| 作者单位： | 1. 中国水产科学研究院,淡水渔业研究中心,农业部水生动物遗传育种和养殖生物学重点开放实验室,无锡,214081
2. 苏州大学医学部基础医学与生物科学学院,苏州,215123 |
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| 基金项目： | 农业部水生动物遗传育种和养殖生物学重点开放实验室开放课题(BM2007-04); 中央级公益性科研院所基本科研业务费专项资金项目(2007JBFB10); 农业行业专项优质蟹种规模化繁育与养殖示范(nyhyzx07-045)资助 |
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| 摘 要： | 为探讨CpG寡脱氧核苷酸(CpG-oligodeoxynucleotides,CpG-ODN)激活中华绒螯蟹血细胞酚氧化酶原系统(prophenoloxidase system,proPO系统)的信号传导途径,使用一定剂量的CpGODN-1670、ODN-R以及几种细胞信号传导的激活剂或抑制剂体外处理中华绒螯蟹血细胞,通过检测胞内外酚氧化酶(PO)活性的变化,对CpG ODN触发proPO激活系统的信号传导途径进行评价。结果显示,ODN-1670与ODN-R均可触发蟹血细胞proPO激活系统,试验剂量的ODN-1670可促进血细胞内外已有的proPO转化为PO,而对proPO颗粒的释放具有一定的抑制作用;ODN-R则不仅可使proPO转化为PO,还可促进血细胞脱颗粒。两者的信号传导途径相似,可能都包含了G-蛋白介导的蛋白激酶C(PKC)途径,酪氨酸蛋白激酶(RTK)途径对ODN-1670的触发proPO激活系统的活化过程进行负调控。
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| 关 键 词： | 中华绒螯蟹 CpG ODNs 酚氧化酶原激活系统 信号传导 |
STUDIES ON THE SIGNALING PATHWAY OF CpG ODNs ACTIVATING proPO SYSTEM IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS |
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| LI Hong-Xia,YU Ju-Hua,LI Yi,XU Pao.STUDIES ON THE SIGNALING PATHWAY OF CpG ODNs ACTIVATING proPO SYSTEM IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS[J].Acta Hydrobiologica Sinica,2011,35(1):122-131. |
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| Authors: | LI Hong-Xia YU Ju-Hua LI Yi XU Pao |
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| Institution: | LI Hong-Xia1,YU Ju-Hua1,LI Yi2 and XU Pao1(1.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China,2.School of Basic Medicine and Bioscience,Medical College of Soochow University,Suzhou 215123,China) |
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| Abstract: | Prophenoloxidase system(proPO system) plays a key role in the natural immune response of Crustacean animals,but the signal transduction pathway of proPO system is rarely reported.CpG-oligodeoxynucleotides(CpG-ODN) is synthetic oligodeoxynucleotide containing CpG motif that could simulate the bacterial DNA with immunostimulatory activity.Many studies has proved that it is a kind of good non-specificity immunostimulants and specific immunology adjuvant.CpG ODNs could stimulate the proPO system in haemocytes o... |
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| Keywords: | Eriocheir sinensis CpG ODNs Prophenoloxidase system Signaling pathway |
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