| 金鱼不同组织器官及胚胎发育不同时期蛋白水解酶的种类和活性变化 |
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| 引用本文： | 李效宇,夏民,李莉,卜艳珍,吉爱玲,姬生栋,徐存拴.金鱼不同组织器官及胚胎发育不同时期蛋白水解酶的种类和活性变化[J].水生生物学报,2000,24(2):128-136. |
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| 作者姓名： | 李效宇 夏民 李莉 卜艳珍 吉爱玲 姬生栋 徐存拴 |
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| 作者单位： | 河南师范大学生物系,河南新乡 453002 |
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| 基金项目： | 中法先进研究计划项目(国科生字[1996]134号)及河南省教委自然科学基金资助项目(编号:21159) |
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| 摘 要： | 采用复性电泳方法研究了金鱼组织器官蛋白水解酶及个体发生过程中蛋白水解酶的种类和活性变化，主要结果表明：⑴金鱼各组织器官蛋白水解酶种类差异不大，大多数组织器官都具有１１３、６９、２０、１６ｋＤ四条带，但不同组织器官常具有其特异性蛋白水解酶；肠道蛋白水解酶种类最多、活性最强。⑵蛋白水解酶的活性受ｐＨ值影响和制约，大多数组织器官蛋白水解酶活性最适ｐＨ值为８．５。⑶在金鱼胚胎发育早期（从卵裂到心跳期）多数
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| 关 键 词： | 金鱼 组织器官 个体发生 蛋白水解酶 胚胎发育 |
| 文章编号： | 1000-3207(2000)02-0128-09 |
| 修稿时间： | 1997年10月2日 |
THE PROTEINASES AND THEIR ACTIVITY IN THE TISSUES AND EMBRYOGENESIS OF GOLDFISH (CARASSIUS AURATUS L.) |
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| LI Xiao-yu, XIA Min, LI Li, BO Yan-zhen, JI Ai-ling, JI Sheng-dong and XU Cun-shuan.THE PROTEINASES AND THEIR ACTIVITY IN THE TISSUES AND EMBRYOGENESIS OF GOLDFISH (CARASSIUS AURATUS L.)[J].Acta Hydrobiologica Sinica,2000,24(2):128-136. |
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| Authors: | LI Xiao-yu XIA Min LI Li BO Yan-zhen JI Ai-ling JI Sheng-dong and XU Cun-shuan |
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| Abstract: | The properties of proteinases in the tissues of goldfish and the kinds of proteinases in the embryogenesis of goldfish were investigated with G-PAGE. The kinds of proteinases were similar, and most tissues of goldfish had four kinds of proteinases with molecular weights being 113 kD, 69 kD, 20 kD and 16 kD respectively.Many kinds of proteinases with strongest activities were observed in the gut. The activities of proteinases were affected by pH, and the optimum pH was 8.5. In the embryogenesis of goldfish, the proteinases began to express at hatching stage, but not in the early embryogenetic period. The proteinases started to express at hatching stage,but most of them expressed at the late developmental stage. |
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| Keywords: | Goldfish (carassius auratus L ) Tissue Ontogenesis Proteinase G-PAGE |
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