西江鲤仔稚鱼生长及消化酶活性变化

为了探究了鲤(Cyprinus carpio)仔稚鱼的生长过程中主要消化酶活性变化规律, 实验测定了鲤从孵化出膜到40 日龄(日龄, Day after hatching)仔稚鱼期间的生长、可溶性蛋白含量和几种消化酶活性变化。结果显示: 仔稚鱼全长以及体重在15 日龄后增速加快, 特定生长率为14.81%。淀粉酶、脂肪酶、胰蛋白酶, 糜乳蛋白酶、碱性磷酸酶以及氨基肽酶在1 日龄仔鱼体内均能检测到活性。在仔稚鱼发育过程中, 其可溶性蛋白含量先下降后上升。仔鱼摄食前消化酶的活性出现一定的上升, 随着仔稚鱼消化系统发育以及营养方式的转变, 其消化酶活性从3到25 日龄处于不断的变化状态, 而25 日龄到实验结束鲤仔稚鱼的消化酶处于一种相对稳定的状态, 标志着其消化功能趋于完善。根据不同发育时期鲤消化酶活性的变化, 设计有机可腐化的材料制备人工鱼巢以提高人工鱼巢的生态修复功能, 降低饥饿对仔稚鱼成活率的影响。

THE GROWTH AND DIGESTIVE ENZYMES ACTIVITIES OF COMMON CARP (CYPRINUS CARPIO) DURING LARVAL STAGE

In order to explore activities of digestive enzymes fluctuation of common carp (Cyprinus carpio) larvae during growth stage, this study investigated the growth, soluble protein content and the activities of digestive enzymes of laboratory-reared common carp. The results indicated that total length and body weight of larvae at 40 days after hatching (DAH) increased greatly with a 14.81% growth rate. The activities of amylase, lipase, trypsin, chymotrypsin, alkaline phosphatase and aminopeptidase were detected at 1 DAH in common carp. During the development of larvae, soluble protein content of larvae decreased firstly, and then gradually increased. The activity of digestive enzymes in larvae increased before feeding. With the development of digestive systems and change of alimentation mode, activities of digestive enzymes of larvae changed continually from 3 to 25 DAH. After 25 DAH, activities of digestive enzymes of common crap were relatively stable, a sign of completed larval digestive function. In order to improve the ecological restoration effect of artificial fishnest and reduce starvation effect on the survival rate of larvae, organic materials could be used for artificial fishnest according to the digestive enzyme activity of common carp at different developmental stages.