| 利用RAPD技术对三种绒螯蟹亲缘关系的研究 |
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| 引用本文： | 谢浩,陆仁后,项超美,张菁,邱涛.利用RAPD技术对三种绒螯蟹亲缘关系的研究[J].水生生物学报,1999,23(2):120-126. |
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| 作者姓名： | 谢浩 陆仁后 项超美 张菁 邱涛 |
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| 作者单位： | 中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072 |
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| 基金项目： | 国家自然科学基金，国家“九五”项目 |
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| 摘 要： | 采用ＲＡＰＤ技术结合一定的形态学研究手段，对中华绒螯蟹，日本绒螯蟹及其合浦亚种进行亲缘关系的研究，为三种绒螯蟹的种及亚种分析重要资料，三种绒螯蟹的第四步足前节长宽比值，与其分类标本吻合或基本吻合，用一组引物，对每种各１０个个体的基因组ＤＮＡ进行扩增，得到一批特异，可重复的扩增图谱，扩增区带的相似率分析表明，中华绒螯蟹与日本绒螯蟹的亲缘关系远，而日本绒螯蟹合浦亚种与日本绒螯蟹近，最后，对文中ＲＡＰＤ
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| 关 键 词： | RAPD 绒螯蟹属 亲缘关系 |
STUDIES ON THE RELATIONSHIP OF THREE KINDS OF MITTEN CRABS USING RAPD TECHNIQUE |
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| Xie Hao, Lu Renhou, Xiang Chaomei, Zhang Jing and Qiu Tao.STUDIES ON THE RELATIONSHIP OF THREE KINDS OF MITTEN CRABS USING RAPD TECHNIQUE[J].Acta Hydrobiologica Sinica,1999,23(2):120-126. |
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| Authors: | Xie Hao Lu Renhou Xiang Chaomei Zhang Jing and Qiu Tao |
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| Abstract: | The RAPD technique, together with the method of morphology, was used to study the genetic relationship among three kinds of important mitten crabs:Eriocheir sinensis, E. japonions and E. japonions hepuensis. The ratios of the length to width of the propodus of the fourth leg in the three kinds of mitten crabs were identical or near to those of taxonomic samples. In RAPD amplification for 10 individuals of every kind of mitten crabs, specific, reproducible bands were yielded.The similarities among the three kinds of crabs showed that the relationship between E.sinensis and E. japonicus is the most distant and that of E. japonions hepuensis and E. japonions is the closest. The sample size of RAPD analysis and the feasibility of RAPD marker for Erioccheir differentiation were discussed. |
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| Keywords: | RAPD Eriocheir Relationship |
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