中华鳖源致病性产吲哚金黄杆菌分离、鉴定及药敏特性分析

对患病中华鳖(Pelodiscus sinensis)进行病原分离、鉴定及药敏实验, 从患病中华鳖皮肤、肝肾脾重要器官分离纯化病原菌, 经理化特性测定及16S rRNA序列分析对其进行鉴定及人工感染试验, 并利用K-B及二倍稀释法进行药敏特性分析。结果表明分离株J22是为中华鳖腐皮病病原, 其对中华鳖的LD₅₀为3.30×104 CFU/g。J22株理化特性与产吲哚金黄杆菌(Chryseobacterium indologenes)一致, 16S rRNA序列与产吲哚金黄杆菌同源性为99%, 综合判定J22株是产吲哚金黄杆菌。分离株对新霉素、庆大霉素及阿莫西林等12种抗生素高度敏感, 对氟苯尼考及多西环素等抗生素耐药; 二氧化氯、漂白粉及高铁酸钾对分离株消毒效果较好。分离菌株J22是中华鳖病原菌, 养殖时可选用庆大霉素、新霉素或者阿莫西林内服, 配合使用二氧化氯、漂白粉及高铁酸钾等外用进行防控。

ISOLATION,IDENTIFICATION AND ANTIBIOTIC SENSITIVITY OF CHRYSEOBACTERIUM INDOLOGENES FROM TRIONYX SINENSIS

This study conducted isolation, identification and drugs sensitivity of pathogen from sick Trionyx sinensis to provide a reference for control and prevention disease for Trionyx sinensis. The pathogenic bacteria were isolated and purified from skin, liver, kidney and spleen of Trionyx sinensis. The identification of the isolated pathogen was analyzed by biochemical reactions and 16S rRNA gene sequence. The artificial infection test was performed with intraperitoneal injection, and drugs susceptibility test was conducted by K-B method and double broth dilution method. The results showed that strain J22 was the pathogen of Trionyx sinensis, and the LD₅₀ was 3.30×104 CFU/g. According to morphological and biochemical characteristics as well as 16S rRNA gene sequence analysis, strain J22 was identified as Chryseobacterium indologenes. Strain J22 was susceptible to neomycin, gentamicin and amoxicillin and other 9 antibiotics but resistant to florfenicol and doxycycline, etc. The chlorine dioxide, bleaching powder and potassium ferrate had great antiseptic effect on the isolated pathogeb. These results indicate that Strain J22-induced disease of Trionyx sinensis can be treat by drugs such as neomycin, gentamicin and amoxicillin for oral administration, and that chlorine dioxide, bleaching powder and potassium ferrate are suitable for external use in farming.