中华绒螯蟹MSTN基因SNPs多态性及与生长性状的关联分析

为研究中华绒螯蟹(Eriocheir sinensis)肌肉生长抑制素基因(myostatin, MSTN)的多态性及其与生长性状的相关性, 对中华绒螯蟹3个群体(育种群体、大赛群体、野生群体)共321个个体MSTN基因的多态性进行筛选, 发现该基因的第1外显子存在3个多态性SNP位点(S1: C714T; S2:G729A; S3:G753T), 均为处于Hardy-Weinberg平衡(P>0.05)的中、高度多态性位点。利用一般线性模型分析3个位点及其基因型组合与生长性状的相关性, 发现S1位点对中华绒螯蟹的体重和壳长等生长性状有显著影响(P≤0.05), 而其余2个位点与生长性状无显著关联性。结果表明S1位点的TT基因型对中华绒螯蟹的生长最为有利, 可作为分子标记辅助育种的候选标记。

POLYMORPHISM OF MSTN GENE AND ITS ASSOCIATION WITH GROWTH TRAITS IN CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS

To study the polymorphism of myostatin (MSTN) gene and its association with growth traits in Chinese mitten crab Eriocheir sinensis, 321 individuals from the three populations (breeding population, contest population and wild population) were screened polymorphic sites of MSTN gene. Three SNPs were found in the first exon of MSTN gene, which were named as S1, S2 and S3, respectively (S1:C714T; S2:G729A; S3:G753T). These three SNPs were middle or high polymorphism loci, and they were all in hardy-Weinberg equilibrium (P>0.05) in population structure. A general linear model used to analyze the correlation between the three SNPs and growth traits (body weight and carapace length) of Chinese mitten crab indicated that S1 but not S2 and S3 had a significant effect on the growth traits (P≤0.05). The TT genotype was the most beneficial genotype on growth of Chinese mitten crab, which could be used as candidate for molecule marker-assisted breeding.