An indexed genomic library for Paramecium complementation cloning |
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| Authors: | Keller A M Cohen J |
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| Institution: | Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France. |
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| Abstract: | Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning. |
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| Keywords: | Complementation cloning hybridization cloning |
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