| 响应面法优化白头翁总黄酮提取工艺 |
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| 引用本文： | 杨爱梅,尚琪,刘轲.响应面法优化白头翁总黄酮提取工艺[J].氨基酸和生物资源,2014,36(3):56-60. |
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| 作者姓名： | 杨爱梅 尚琪 刘轲 |
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| 作者单位： | 兰州理工大学生命科学与工程学院,甘肃兰州,730050 |
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| 摘 要： | 采用无水乙醇C2H5OH/硫酸铵(NH4)2SO4双水相体系分离白头翁中的黄酮。确定双水相体系组成为21%C2H5OH/22%(NH4)2SO4,通过单因素试验和Box-Benhnken实验设计探讨黄酮粗提液质量分数、NaCl质量分数和pH值对萃取效果的影响。确定最佳萃取条件为:黄酮粗提液质量分数12.5%,NaCl质量分数1.5%,pH 5.99,在此条件下,白头翁总黄酮主要分布在上相,萃取率可达73.6%。
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| 关 键 词： | 双水相体系 响应面法 白头翁 黄酮 |
Optimization of Extraction Technology of Total Flavonoid from Pulsatilla chinensis by Response Surface Analysis |
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| YANG Aimei,SHANG Qi,LIU Ke.Optimization of Extraction Technology of Total Flavonoid from Pulsatilla chinensis by Response Surface Analysis[J].Amino Acids & Biotic Resources,2014,36(3):56-60. |
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| Authors: | YANG Aimei SHANG Qi LIU Ke |
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| Institution: | ,College of Life Science and Engineering,Lanzhou University of Technology |
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| Abstract: | An aqueous two-phase system composed of 21%C2H5OH and 22%(NH4)2SO4was used to extract total flavonoid fromPulsatilla chinensis.The effects of crude extract mass fraction,NaCl mass fraction and pH value on the extraction efficiency of total flavonoid were explored by one-factor-at-a-time and Box-Benhnken design methods.The optimal aqueous two-phase extraction conditions were crude extract mass fraction of 12.5%,NaCl mass fraction of 1.5%and pH of 5.99.Under the optimal extraction conditions,total flavonoid fromPulsatilla chinensis root was mainly distributed in the upper phase with an extraction rate of 73.6%. |
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| Keywords: | aqueous two-phase system response surface method Pulsatilla chinensis flavonoid |
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