| 通过基因工程构建谷氨酸生产菌B44的研究 |
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| 引用本文： | 周盛,李家洲,武波.通过基因工程构建谷氨酸生产菌B44的研究[J].氨基酸和生物资源,2006,28(1):33-36. |
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| 作者姓名： | 周盛 李家洲 武波 |
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| 作者单位： | 1. 广西玉林师范学院化生系,玉林,537000;2. 广西大学生命科学院 |
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| 基金项目： | 广西玉林师范院重点科研项目(编号05ZDHS03)资助。 |
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| 摘 要： | 从大肠杆菌E.colik-12中通过PCR克隆出磷酸果糖激酶编码基因(pfkA),将其连到表达载体pCMVTNTTMvector。连接构建成重组质粒Ku-1,导入谷氨酸棒杆菌B4(已经诱变改造),并得到表达。酶活性测定表明Ku-1的pfkA基因在B44中得到表达(磷酸果糖激酶为128.6±0.86U/g蛋白)。解除磷酸果糖激酶对已经改造的谷氨酸的整个代谢途径的限制。同时,B44对糖转化率比B4(由出发菌株B1诱变而来)高10.64%,产酸率比B4高17.1%。
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| 关 键 词： | PCR克隆 表达载体 代谢途径 |
| 文章编号： | 1006-8376(2006)01-0033-04 |
| 收稿时间： | 12 25 2005 12:00AM |
| 修稿时间： | 2005年12月25日 |
Study on Constructing Corynebacterium glutamicum B44 by Gene Engineering |
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| ZHOU-sheng,LI Jia-zhou,WU Bo.Study on Constructing Corynebacterium glutamicum B44 by Gene Engineering[J].Amino Acids & Biotic Resources,2006,28(1):33-36. |
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| Authors: | ZHOU-sheng LI Jia-zhou WU Bo |
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| Institution: | ZHOU-sheng~1,LI Jia-zhou~1 WU Bo~2 |
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| Abstract: | Phosphofructokinase-1(EC.2.7.1.11)gene from E.coli K-12 was amplified by PCR and inserted into plasmid pCMVT_NT~(TM) to create a reoombinant plasmid Ku-1.Then the plasmid was introduced into B4 and expressed.The pfkA gene was expressed in B44 strain \.The restraint of the whole passway of Glu metabolism was removed by increasing Phosphfructokinase-1.At the same time,the strain B44 showed respectively a glutamate productivity of 17.1% higher and a glutamatr/glucose conversion rate of 10.64% higher than those of starting strain B4. |
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| Keywords: | PCR cloning expression vecter metabolic passway |
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