| 四种氨基酸原料细菌内毒素检查法的研究 |
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| 引用本文： | 杨善彬,梅虎,孙宇阳,李志良.四种氨基酸原料细菌内毒素检查法的研究[J].氨基酸和生物资源,2007,29(2):70-72. |
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| 作者姓名： | 杨善彬 梅虎 孙宇阳 李志良 |
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| 作者单位： | 重庆大学,生物工程学院,重庆,400044;重庆大学,生物医学工程教育部与重庆市重点实验室,重庆,400044;宜宾学院,生物工程系,四川,宜宾,644000;重庆大学,生物工程学院,重庆,400044;重庆大学,生物医学工程教育部与重庆市重点实验室,重庆,400044;重庆师范大学,化学院,重庆,400030 |
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| 摘 要： | 依据中国药典2000年版“细菌内毒素检查法”,通过鲎试剂的干扰试验,研究注射用氨基酸原料中细菌内毒素检查法的可行性。结果在2~16倍稀释级下,L-缬氨酸(c=2.5%)和L-异亮氨酸(c=2.5%)对鲎试剂无干扰,检测细菌内毒素的鲎试剂灵敏度≤5.0×102EU.L-1,而在16倍稀释级范围内,L-脯氨酸(c=4.5%)和L-亮氨酸(c=2.0%)对鲎试剂检查法有抑制作用。结论为注射用L-异亮氨酸和L-缬氨酸用灵敏度为5.0×102EU.L-1的鲎试剂检查其细菌内毒素方法可行,可以代替家兔法检查热原。
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| 关 键 词： | L-脯氨酸 L-缬氨酸 L-亮氨酸 L-异亮氨酸 细菌内毒素检查 干扰试验 鲎试剂 |
| 文章编号： | 1006-8376（2007）02-0070-03 |
Study of Bacterial Endotoxin Test (BET) for Four Individual Amino Acids |
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| YANG Shan-bin,MEI Hu,SUN Yu-yang,LI Zhi-liang.Study of Bacterial Endotoxin Test (BET) for Four Individual Amino Acids[J].Amino Acids & Biotic Resources,2007,29(2):70-72. |
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| Authors: | YANG Shan-bin MEI Hu SUN Yu-yang LI Zhi-liang |
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| Institution: | 1. College of BIO - engineering, Chongqing University, Chongqing 400044 ; 2. Key Laboratory of Biomedical Engineering of Educational Ministry and Chongqing City, Chongqing 400044; 3. Department of Bio -engineering, Yibin University, Yibin 644000; 4. College of Chemistry, Chongqing Normal University, Chongqing 400030, China |
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| Abstract: | In studying the feasibility of the method for examination of bacterial endotoxin in amino acids in injection by tachypleus amebocytelysate(TAL) interference test,L-Valine(c=2.5%) and L-isoleucine(c=2.5%) did not interfere with TAL reagent in 2-16 times diluted concentrations and it could be tested with TAL that sensitivity was 0.5EU.ml-1.But L-proline(c=4.5%) and L-leucine(c=2.0%) had inhibition with TAL under 16 times diluted scales.The method of bacterial endotoxin test of L-valine and L-isoleucine in injection was feasible,and could replace the rabbit test for pyrogen. |
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| Keywords: | L-proline L-leucine L-valine L-isoleucine bacterial endotoxin test interference test TAL reagent |
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