蛋氨酸羟基类似物对肉鸡骨骼性能和基因表达的影响

本文以雄性罗斯肉鸡为材料,探讨饲粮添加不同蛋氨酸源:蛋氨酸(DLM)、蛋氨酸羟基类似物(HMTBA)和蛋氨酸羟基类似物钙盐(HMTB-Ca)对肉鸡骨骼性能和基因表达的影响。结果表明,HMTBA和HMTB-Ca能提高肉鸡终体重、日增重、血液GSH/GSSG比值、抗氧化酶(CAT)水平、总抗氧化能力(T-AOC)、骨骼长度、重量、骨骼指数(p<0.05)。不同蛋氨酸源对肉仔鸡采食量、料重比和灰分含量影响不显著(p>0.05),HMTBA组骨生长分化因子-5(GDF-5)表达量显著上升(p<0.05)。HMTB-Ca显著提高骨骼强度、钙磷含量,下调金属基质蛋白酶-2(MMP-2)、金属基质蛋白酶-9(MMP-9)的表达水平(p<0.05)。由此可知,采食蛋氨酸羟基类似物及其钙盐饲粮,可提高肉鸡体增重,改善血液氧化还原状态,改善骨骼生长发育,其中HMTB-Ca对骨骼作用更显著。

Effect of Methionine Hydroxy Analogue on Bone Function and Gene Expression of Broiler Chickens

The purpose of this study is to investigate the effects of diets supplemented with three different methionine sources DL-Met(DLM),2-hydroxy-4-(methythio) butanoic acid(HMTBA) and methionine hydroxy analog-casalt(HMTB-Ca) on bone development and gene expression of broiler chicken.The results showd that,HMTBA HMTB-Ca significantly improved broiler daily weight gain,plasma GSH/GSSG ratio,CAT levels,T-AOC levels,bone length and weight,bone index(p<0.05).Examination of daily feed intake,feed efficiency and ash revealed no difference amongst dietary treatments(p>0.05),HMTBA significantly increased growth differentiation factor-5 expression levels(p<0.05) while HMTB-Ca significantly improved bone strength,calcium and phosphorus,and significantly decreased metal matrix metalloproteinase-2,metal matrix metalloproteinase-9 expression levels(p<0.05).In conclusion,broilers fed with methionine hydroxy analogue could increase daily weight gain and plasma antioxidant levels,improve bone growth and development especially when HMTB-Ca was fed.