| 重组酶法定量分析吡咯喹啉醌 |
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| 引用本文： | 丁远春,李志红.重组酶法定量分析吡咯喹啉醌[J].氨基酸和生物资源,1997,19(4):17-20. |
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| 作者姓名： | 丁远春 李志红 |
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| 作者单位： | 武汉大学生命科学院 |
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| 摘 要： | 用ＤＥＡＥ－ｓｅｐｈａｃｅｌ和ＣＭ－ｃｅｌｕｌｏｓｅ柱层析的方法从Ｃｏｍａｍｏｎａｓｔｅｓｔｏｓｔｅｒｏｎｉ菌体中纯化得到一定纯度的脱辅基的乙醇脱氢酶。在含３ｍＭＣａＣｌ２的Ｔｒｉｓ／ＨＣｌ缓冲液（２０ｍＭ，ｐＨ７．０）中，该酶能与ＰＱＱ重组成有活性的全酶，测出的全酶活性大小与外加ＰＱＱ的量成正比，从而定量分析ＰＱＱ。该法专一、灵敏、可靠。
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| 关 键 词： | 脱辅基的乙醇脱氢酶 重组 吡咯喹啉醌 |
Method of Enzymatic Determination of Pyrroloquinoline Quinone |
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| Ding Yuanchun,Li Zhihong He Jianshe Zhao Yongfang.Method of Enzymatic Determination of Pyrroloquinoline Quinone[J].Amino Acids & Biotic Resources,1997,19(4):17-20. |
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| Authors: | Ding Yuanchun Li Zhihong He Jianshe Zhao Yongfang |
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| Abstract: | Alcohol dehydrogenase apoenzyme was purified partially from comamonas testosteroni with DEAE-sephacel column and CM-cellulose column, In a Tris/HCL buffer (20mM.pH7.0)containing 3mM CaCl 2,a holoenzyme can be reconstituted with pQQ and the apoenzyme. Morover, the maeasured activity of holoenzyme was in direct proportion to the quantity of added exogenous pQQ. So assaying the restored enzyme activity gave a quantitative determination of Pyrroloquinoline Quinone. The Method was confirmed to be sensitive, specifical and reliable |
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| Keywords: | Alcohol Dehydrogenase Apoenzyme Reconstitution Pyrroloqunoline Quinone |
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