| 反相高效液相色谱法检测脂必妥片中洛伐他汀的含量 |
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| 引用本文： | 陈志强,卓瑞,金杨,张明辉.反相高效液相色谱法检测脂必妥片中洛伐他汀的含量[J].氨基酸和生物资源,2008,30(3):74-76. |
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| 作者姓名： | 陈志强 卓瑞 金杨 张明辉 |
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| 作者单位： | 天津市尖峰天然产物研究开发有限公司,天津,300457;天津科技大学食品工程与生物技术学院食品生物技术研究室,教育部食品营养与安全重点实验室,天津,300457 |
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| 基金项目： | 天津科技大学引进人才科研启动基金 |
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| 摘 要： | 对脂必妥片样品进行预处理,得到的混合物用薄层色谱、反相高效液相色谱法进行定性、定量分析,建立检测其含量的方法。当色谱条件为色谱柱:Kromasil(C18250 mm×4.6 mm,5μm);流动相体积比:乙腈∶水为85∶15,;流速:1.0 ml.min-1;检测波长:420 nm;柱温:30℃;测定结果表明被测峰和其它峰可完全分离,在每毫升10.21~200.03μg内具有良好的线性关系,r=0.9997,测得其中洛伐他汀含量为0.25%,回收率:97.73%,RSD:0.56%。这种方法准确、可靠,可用于含洛伐他汀药品的质量控制。
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| 关 键 词： | 脂必妥片 洛伐他汀 反相高效液相色谱 薄层色谱 |
The Quantitative Analysis of Lovastatin in Zhibituo Tablet With RP- HPLC |
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| CHEN Zhi-qiang,ZHUO Rui,JIN Yang,ZHANG Ming-hui.The Quantitative Analysis of Lovastatin in Zhibituo Tablet With RP- HPLC[J].Amino Acids & Biotic Resources,2008,30(3):74-76. |
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| Authors: | CHEN Zhi-qiang ZHUO Rui JIN Yang ZHANG Ming-hui |
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| Abstract: | Carrying on the pretreatment to the sample of Zhibituo tablet,then TLC and RP-HPLC was selected to analyze quality and quantity for lovastatin.Method RP-HPLC was used with standard lovastatin as reference substances.The chromatography condition was: Kromasil(C18 250mm ×4.6mm,5μm),a mixture of methanol and water(85:15,V/V) as mobile phase,flow rate of 1.0mL·min-1,detecting wavelength at 420nm,column temperature: 30℃.The results indicated that the apex of survey and the others could be separated completely.A good linearity was shown in the concentration range of 10.21～200.03μg·mL-1(r=0.9997).The content of lovastatin was 0.247%.The average recovery was 97.73%,RSD 0.56%.The method was simple and accurate.t could be used for quality control of lovastatin. |
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| Keywords: | Zhibituo tablet lovastatin RP-HPLC TLC |
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