| 苯丙氨酸的脱氨酶法测定 |
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| 引用本文： | 范长胜,胡宝龙.苯丙氨酸的脱氨酶法测定[J].氨基酸和生物资源,1995,17(3):33-35. |
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| 作者姓名： | 范长胜 胡宝龙 |
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| 作者单位： | 复旦大学微生物学系 |
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| 摘 要： | 方法的原理是：由某些微生物所产生的苯丙氨酸脱氨酶能使苯丙氨酸分子氧化脱氨，生成相应的苯丙酮酸。在合适的反应体系中，苯丙酮酸与三氯化铁反应生成的化合物呈蓝绿色，这种颜色的深浅与苯丙氨酸含量成正比，制备的标准曲线有良好的线性。因而可借此法对测试样品中的苯丙氨酸含量
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| 关 键 词： | 苯丙氨酸脱氨酶，苯丙氨酸，苯丙酮酸，三氯化铁 |
Determination of Phenylalanine by Deaminase |
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| Fan Changsheng,HuBaolong,Yang Rengen.Determination of Phenylalanine by Deaminase[J].Amino Acids & Biotic Resources,1995,17(3):33-35. |
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| Authors: | Fan Changsheng HuBaolong Yang Rengen |
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| Institution: | ,Fan Changsheng,HuBaolong,Yang Rengen(Department of Microbiology, Fudan University Shanghai 200433) |
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| Abstract: | This method is used for determination of phenylalanine in solution.It is based on that phenylalanine-deaminase, extracted from microbes, catalyzes the deamination of L-phenylalanine to produce phenylpyruvate. Then the phenyl-pyruvate reacts with ferric chloride to form a compound of blue-green colour. In suitable reaction system, various shades of the colour direactly proportional are sdirectly proportional to the concentrations of phenylalanine. |
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| Keywords: | Phenylalanine-deaminase Phenyrlalnine Phenyrlpyruvate Ferric chloride |
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