| 比色法测定复合氨基酸注射液中的N-乙酰-L-半胱氨酸 |
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| 引用本文： | 靳继德,童旭彬.比色法测定复合氨基酸注射液中的N-乙酰-L-半胱氨酸[J].氨基酸和生物资源,1999,21(2):4-6. |
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| 作者姓名： | 靳继德 童旭彬 |
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| 作者单位： | [1]北京放射医学研究所 [2]广州第一军医大学 |
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| 摘 要： | 根据Ｎ－乙酰－Ｌ－半胱氨酸与氯化钯（ＰｄＣｌ２）反应，生成一种稳定的黄色化合物，我们建立了比色测定复合结晶氨基酸注射液中的ＮＡＣ的方法。测定波长为３７５ｎｍ，检测结果重现性好，变异系数是３．９％，回收率范围在９３．１％～１００．２％之间，检测下限为０．１６３μｇ／ｍｌ。该方法比经典的电量滴定法灵敏度高、精确性好，较高压液相色谱法操作简便、费用低。
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| 关 键 词： | 比色法 N－乙酰－L－半胱氨酸 复合结晶氨基酸注射液 |
Colorimetric Determination of N Acetylcysteine in Crystalline Amino Acid Solution |
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| Jin Jide Xu Qishou Tong Xubin.Colorimetric Determination of N Acetylcysteine in Crystalline Amino Acid Solution[J].Amino Acids & Biotic Resources,1999,21(2):4-6. |
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| Authors: | Jin Jide Xu Qishou Tong Xubin |
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| Institution: | Institute of radiation midcine Beijing 100850 |
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| Abstract: | A colorimetric determination of N acetylcysteine(NAC) in crystalline amino acid solution was developed,based on the fact that NAC reacts with palladium chloride(PdCl 2) and forms a stable yellow complex which could be colorimetrically determined at wavelength of 375nm. The coefficient of variation was 3.9%. The yield of recovery was 93.1%～100.2%, and the lowest detection limits was 0.163μg/ml. The method had good sensitivity and accuracy compared with coulometric titration. The method was simpler and more economical compared with high-perfomance liquid chromatography. |
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| Keywords: | Colorimetry N-Acetylcysteine Crystalline Amino Acids Solution |
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