| 宁康霉素产生菌海洋地衣芽孢杆菌BAC-9912的诱变育种 |
| |
| 引用本文： | 孙冬雪,胡江春.宁康霉素产生菌海洋地衣芽孢杆菌BAC-9912的诱变育种[J].氨基酸和生物资源,2005,27(4):19-22. |
| |
| 作者姓名： | 孙冬雪 胡江春 |
| |
| 作者单位： | 沈阳药科大学,辽宁,沈阳,110016;中国科学院沈阳应用生态研究所,辽宁,沈阳,110016 |
| |
| 基金项目： | 中国科学院知识创新工程重要方向项目（KSCX2-SW-301-07） |
| |
| 摘 要： | 宁康霉素(Nincomycin)产生菌BAC-9912经紫外线单因子和亚硝基胍化学试剂诱变后,获得两株高产菌株9912-7-2U(UV处理获得)和9912-2U-32N(NTG处理获得),其摇瓶效价分别为176.464×105u·L-1和217.808×105u·L-1。比出发菌株效价分别提高126.9%和180.0%。连续传代摇瓶效价稳定。初步建立了形态观察法和琼脂块大通量法两种初筛模型。
|
| 关 键 词： | 地衣芽孢杆菌 紫外诱变 亚硝基胍 琼脂块大通量法 二计量法 |
| 文章编号： | 1006-8376(2005)04-0019-04 |
| 收稿时间： | 09 21 2005 12:00AM |
| 修稿时间： | 2005年9月21日 |
Mutagenesis and Strain-Improving of Nincomycin-Producing Strain Marine Bacteria BAC-9912 |
| |
| SUN Dong-xue,HU Jiang-chun.Mutagenesis and Strain-Improving of Nincomycin-Producing Strain Marine Bacteria BAC-9912[J].Amino Acids & Biotic Resources,2005,27(4):19-22. |
| |
| Authors: | SUN Dong-xue HU Jiang-chun |
| |
| Institution: | SUN Dong-xue~ HU Jiang-chun~ |
| |
| Abstract: | Nincomycin-producer, the marine bacteria BAC-9912 was mutagenized with UV-irradiation and NTG. Two high yield strains 9912-72U(obtained from UV-irradiation) and 9912-2U-32N(obtained by NTG) were obtained. The fermentation potency of two stains 9912-7-2U and 9912-2U-32N in shaker were 176.464×10~5u·L~-1 and 217.808×10~5u·L~-1, the yield increased 126.9% and 180.0% as compared with parent strain. The productivities of two mutant strains were stable. Two models of piece of agar largest through-put screen and colony form screen were established. |
| |
| Keywords: | UV - irradiation mutagenesis NTG piece of agar largest screen method of two dosage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
