| 正交设计优选橙皮苷提取工艺研究 |
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| 引用本文： | 赵琴,李亚丹.正交设计优选橙皮苷提取工艺研究[J].氨基酸和生物资源,2010,32(3):45-48. |
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| 作者姓名： | 赵琴 李亚丹 |
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| 作者单位： | 湖南农业大学动物医学院,湖南,长沙,410128;湖南农业大学,湖南省农业生物工程研究所,湖南,长沙,410128;湖南农业大学,湖南省农业生物工程研究所,湖南,长沙,410128 |
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| 基金项目： | 湖南农业大学青年科学基金 |
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| 摘 要： | 采用碱-醇提酸沉法提取陈皮中橙皮苷,首先针对提取温度、提取时间、pH值、碱液料液比、醇液料液比、提取次数等因素对提取率的影响进行单因素实验考察,并进一步利用正交实验对橙皮苷的提取工艺进行优化。得到最优浸提条件为:陈皮粉与食用乙醇料液比为1:10、与0.5%NaOH溶液料液比1:10,浸提温度70℃,浸提时间4 h,提取率最高。
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| 关 键 词： | 橙皮苷 碱-醇提酸沉法 提取工艺 |
Optimization of Extracting Hesperidin from Orange Peel by Orthogonal Design |
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| ZHAO Qin,LI Ya-dan.Optimization of Extracting Hesperidin from Orange Peel by Orthogonal Design[J].Amino Acids & Biotic Resources,2010,32(3):45-48. |
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| Authors: | ZHAO Qin LI Ya-dan |
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| Institution: | ZHAO Qin1,2,LI Ya-dan2(1.Animal Medical College,Hunan Agricultural University,Changsha 410128,2.Agricultural and Bioengineering Institute of Hunan Province,China) |
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| Abstract: | Extraction technology of hesperidin from orange peel was studied by using ethanol-alkali extraction and acid precipitation method.Extraction temperature,pH,the rate of NaOH to materials,the rate of CH3CH2OH to matorial were studied on the base of these single factors for optimization.The rusults showed that ethanol-alkali extraction and acid precipitation gave the best effect when the rate of NaOH to material was 10:1,the rate of CH3CH2OH to material was 10:1,extracted at 70℃ for 4 hours. |
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| Keywords: | hesperidin extraction technology ethanol-alkali extraction acid precipitation method |
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