| 红酵母细胞中β-胡萝卜素的快速分离柱高效液相色谱法测定 |
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| 引用本文： | 朱惠贤,王林,章新,杨光宇,胡秋芬.红酵母细胞中β-胡萝卜素的快速分离柱高效液相色谱法测定[J].氨基酸和生物资源,2004,26(4):74-76. |
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| 作者姓名： | 朱惠贤 王林 章新 杨光宇 胡秋芬 |
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| 作者单位： | 玉溪师范学院化学与环境科学系,玉溪,653100;玉溪师范学院化学与环境科学系,玉溪,653100;玉溪师范学院化学与环境科学系,玉溪,653100;玉溪师范学院化学与环境科学系,玉溪,653100;玉溪师范学院化学与环境科学系,玉溪,653100 |
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| 基金项目： | 玉溪师范学院校科研和教改项目，云南省自然科学基金 |
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| 摘 要： | 研究了用快速分离柱高效液相色谱法测定红酵母细胞中β-胡萝卜素的方法。发酵法得到的样品经匀浆后用四氢呋喃提取,提取液以ZORBAXStableBound(4.6×50mm,1.8μm)C18快速分离柱为固定相,乙腈-四氢呋喃体积比8020为流动相分离,流速为1.5ml/min;在该色谱条件下,β-胡萝卜素在1.0min内可达到基线分离;用紫外二极管矩阵检测器检测。方法标准回收率为98%～103%,相对标准偏差为0.46%～0.58%,可用于几种发酵样品中β-胡萝卜素的测定,结果可靠。
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| 关 键 词： | 高效液相色谱 β-胡萝卜素 红酵母 |
| 文章编号： | 1006-8376(2004)04-0074-03 |
| 修稿时间： | 2004年8月21日 |
Determination of β-Carotene in Red Yeast by Rapid Column High Performance Liquid Chromatography |
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| ZHU Hui-xian,WANG Lin,ZHANG Xin,YANG Guang-yu,HU Qiu-fen.Determination of β-Carotene in Red Yeast by Rapid Column High Performance Liquid Chromatography[J].Amino Acids & Biotic Resources,2004,26(4):74-76. |
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| Authors: | ZHU Hui-xian WANG Lin ZHANG Xin YANG Guang-yu HU Qiu-fen |
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| Abstract: | A rapid column high performance liquid chromatography (HPLC) method for determination of β-carotene in red yeast (Rhodotorula glutinis) was studied. The β-carotene was extracted from the sample with tetrahydrofuran by ultrasonic extraction. Then the β-carotene was separated on a ZORBAX Stable Bound (4.6×50 mm, 1.8μm) rapid chromatographic column with acetonitrile-tetrahydrofuran (80:20) as mobile phase at a flow-rate of 1.5 ml·min -1 , and monitored with the photodiode array detector at 451 nm. The recoveries of the β-carotene were 98% to 103%, and the relative standard deviations were 0.46% to 0.58%. This method was applied to the determination of β-carotene in red yeast with good results. |
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| Keywords: | high performance liquid chromatography β-carotene red yeast |
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