| 辣木资源利用中的悬浮细胞培养体系研究 |
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| 引用本文： | 岳涵,覃瑞,龚汉雨,宋雪杨,熊海容,刘虹,余光辉.辣木资源利用中的悬浮细胞培养体系研究[J].氨基酸和生物资源,2018(1):31-35. |
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| 作者姓名： | 岳涵 覃瑞 龚汉雨 宋雪杨 熊海容 刘虹 余光辉 |
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| 作者单位： | 武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉,430074
中南民族大学生命科学学院,湖北武汉,430074
武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉430074;中南民族大学生命科学学院,湖北武汉430074 |
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| 基金项目： | 科技部基础性工作专项，中央高校基本科研业务费培育类项目，国家自然科学基金 |
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| 摘 要： | 辣木富含多种营养成分,在食品和药物开发方面有巨大的潜在开发价值。本文提供了一种可行的辣木细胞悬浮培养技术。由辣木的根诱导形成愈伤组织和叶诱导形成愈伤组织的合适细胞悬浮培养条件分别为MS培养基(MS)+1.0mg/L 2,4-二氯苯氧乙酸(2,4-D)+1.0mg/L激动素(KT)和MS+0.5mg/L 2,4-D+0.5mg/L KT,摇床转速均为50~100r/min,将愈伤组织添加到液体悬浮培养基中20d左右可得到大量悬浮细胞。本研究为辣木细胞水平的培养和研究提供了一条途径,为辣木潜在价值的开发利用提供新的思路。
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| 关 键 词： | 辣木 愈伤组织 细胞悬浮培养 |
Suspension cell culture of Moringa oleifera for resource utilization |
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| YUE Han,QIN Rui,GONG Hanyu,SONG Xueyang,XIONG Hairong,LIU Hong,YU Guanghui.Suspension cell culture of Moringa oleifera for resource utilization[J].Amino Acids & Biotic Resources,2018(1):31-35. |
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| Authors: | YUE Han QIN Rui GONG Hanyu SONG Xueyang XIONG Hairong LIU Hong YU Guanghui |
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| Institution: | ,Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China,College of Life Sciences,South-Central University for Nationalities |
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| Abstract: | Moringa oleifera has great potential on food and drug development,owning to its rich nutrients.This paper provides a feasible cell suspension culture technology.The conditions of cell suspension culture for callus induced by root and leaf were MS medium (MS)+1.0 mg/L (2,4-dichlorophenoxy) acetic acid (2,4-D)+ 1.0 mg/L kinetin (KT) and MS+0.5 mg/L 2,4-D-+-0.5 mg/L KT,respectively.The rotating speed was 50～100 r/min.After adding the callus to liquid suspension culture medium for about 20 days,a large number of suspension cells was obtained.This study provides a new way to culture and research the Moringa oleifera in the cell level,as well as new ideas for the development and utilization of the potential value of Moringa. |
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| Keywords: | Moringa oleifera callus cell suspension culture |
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