| 响应面法优化超声提取苦瓜皂苷工艺条件的研究 |
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| 引用本文： | 陈志强,李贞景,王昌禄.响应面法优化超声提取苦瓜皂苷工艺条件的研究[J].氨基酸和生物资源,2007,29(4):21-25. |
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| 作者姓名： | 陈志强 李贞景 王昌禄 |
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| 作者单位： | 天津科技大学,食品工程与生物技术学院,食品生物技术研究室,天津,300457;天津市尖峰天然产物研究开发有限公司,天津,300457;天津科技大学,食品工程与生物技术学院,食品生物技术研究室,天津,300457 |
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| 摘 要： | 利用响应面法对超声提取苦瓜皂苷的工艺条件进行了优化。在对超声时间、超声温度、超声功率、料液比等单因子实验的基础上,根据中心组合设计原理采用四因子三水平的响应面分析法,通过对各因子显著性和交互作用的分析,得出了超声提取苦瓜皂苷的最佳工艺条件为:超声时间41 min,超声温度62℃,超声功率402 w,料液比1:23,此时苦瓜皂苷的实际得率为3.03%。
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| 关 键 词： | 响应面法 提取 苦瓜 总皂苷 |
| 文章编号： | 1006-8376（2007）04-0021-05 |
| 收稿时间： | 2007-09-09 |
| 修稿时间： | 2007年9月9日 |
Study on Optimum Technique of Extracting Total Saponins from Bitter Melon by Supersonic Wave |
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| CHEN Zhi-qiang,LI Zhen-jing,WANG Chang-lu.Study on Optimum Technique of Extracting Total Saponins from Bitter Melon by Supersonic Wave[J].Amino Acids & Biotic Resources,2007,29(4):21-25. |
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| Authors: | CHEN Zhi-qiang LI Zhen-jing WANG Chang-lu |
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| Abstract: | To optimize the extraction technique for total saponins from bitter melon by supersonic wave,the single factor tests and response surface method(RSM) were used.On the basis of the previous results and according to the central composite experimental design principles,the response surface method with 4 factors and 3 levels was adopted.The results showed that the optimum conditions were that supersonic wave time of 41 min,supersonic power of 402 W,extracting temperature of 62℃ and the ratio of bitter melon to solvent of 1:23. Under such conditions the extraction rate of total saponins could be up to 3.03% at one-time extraction. |
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| Keywords: | response surface method(RSM) extraction bitter melon total saponins |
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