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乙醇-磷酸氢二钾双水相体系萃取L-精氨酸
引用本文:刘东梅,翁连进,耿頔,杨欣,韩媛媛.乙醇-磷酸氢二钾双水相体系萃取L-精氨酸[J].氨基酸和生物资源,2013,35(1):31-34.
作者姓名:刘东梅  翁连进  耿頔  杨欣  韩媛媛
作者单位:华侨大学化工学院,福建厦门,361021;华侨大学化工学院,福建厦门,361021;华侨大学化工学院,福建厦门,361021;华侨大学化工学院,福建厦门,361021;华侨大学化工学院,福建厦门,361021
基金项目:中央高校基本科研业务费资助项目
摘    要:采用乙醇-磷酸氢二钾(K2HPO4)双水相体系萃取L-精氨酸。实验考察了乙醇浓度、K2HPO4浓度、pH、萃取温度对萃取分离L-精氨酸的影响。结果表明,L-精氨酸在该双水相体系的分配系数K随体系乙醇浓度、K2HPO4浓度的增大、萃取温度的升高而增大,随着体系pH的增大而减小;L-精氨酸在该双水相体系的萃取率随体系乙醇浓度和pH的增大而减小,随着体系K2HPO4浓度增大、萃取温度的升高而增大。

关 键 词:双水相萃取  L-精氨酸  分配系数  萃取率

Extraction of L-Arginine by Aqueous Two-Phase Systems Containing Ethanol and Dipotassium Hydrogen Phosphate
LIU Dongmei , WENG Lianjin , GENG Di , YANG Xin , HAN Yuanyuan.Extraction of L-Arginine by Aqueous Two-Phase Systems Containing Ethanol and Dipotassium Hydrogen Phosphate[J].Amino Acids & Biotic Resources,2013,35(1):31-34.
Authors:LIU Dongmei  WENG Lianjin  GENG Di  YANG Xin  HAN Yuanyuan
Institution:(College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian, China)
Abstract:The ethanol-dipotassium hydrogen phosphate aqueous two-phase system was used to extract L-arginine. The influences of concentration of ethanol and dipotassium hydrogen phosphate, pH value and temperature on extraction were discussed. The results indicated that the partition coefficient K of L-arginine increased with the increasing of concentration of ethanol and dipotassium hydrogen phosphate and the rise of temperature, decreased with the increasing of pH value; The rate of extraction of L-arginine decreased with the increasing of concentration of ethanol and pH value, increased with the increasing of concentration of dipotassium hydrogen phosphate and the rise of temperature.
Keywords:aqueous two-phase extraction  L-arginine  partition coefficient  rate of extraction
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