不同居群五柱绞股蓝叶形变异及总黄酮含量分析

以不同生境的五柱绞股蓝为材料,对4个不同来源的五柱绞股蓝的叶片性状、裂叶数进行了统计,并以芦丁为对照品,采用分光光度法对4个居群五柱绞股蓝的总黄酮含量进行了测定。结果表明,五柱绞股蓝的叶型变异较大,其复叶具有3-9裂叶型类型,以7裂叶型和5裂叶型为主。相关性统计显示,野生型五柱绞股蓝黄酮含量高,与7裂叶型极显著相关,家种型五柱绞股蓝黄酮含量低,与叶型无关,环境因子可能是影响其黄酮含量的主要因子。在引种时,7裂叶型的五柱绞股蓝可作为优选资源引用。

Study on Leaf Shape Variation and Total Flavonoids in Different Populations of Gynostemma pentagynum Z.P.Wang

Leaf features,cleft leaf number and total flavonoids in four populations of Gynostemma pentagynum Z.P.Wang were investigated.Rutin was used as a reference substance,spectrophotometry was employed to determine the total flavonoids of G.pentagynum.The results showed that the G.pentagynumleaf type was variable,the cleft leaf number varied from 3to 9,and mainly dominated by 7and 5.Wild type total flavonoid contents were higher than that of cultivated type and have a significant positive correlation with 7-cleft-leaf.However,there was no significant correlation between flavonoid and leaf type in cultivated type.It suggested that environmental factors may be the primary factor affecting the flavonoid content.The 7-cleft-leaf G.pentagynumcould be one of optimal resources.