| 透明质酸产生菌的选育及发酵培养基的优化 |
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| 引用本文： | 吴明霞,邓静,吴华昌,魏岱旭,陈艺德.透明质酸产生菌的选育及发酵培养基的优化[J].氨基酸和生物资源,2008,30(1):37-40. |
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| 作者姓名： | 吴明霞 邓静 吴华昌 魏岱旭 陈艺德 |
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| 作者单位： | 四川理工学院,四川,自贡,643000 |
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| 基金项目： | 四川省科技厅科技计划项目，四川省应用基础研究项目 |
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| 摘 要： | 将实验室保存UVD-34透明质酸产生菌再进行超声波处理,挑选出了一株透明质酸产量较高的菌,并对其发酵培养基进行初步优化。结果表明当可溶性淀粉质量分数为2%,复合氮源为3%,碳酸氢钠为0.1%时,其透明质酸的产量可达4.59 g.L-1,比优化前提高了1.29倍。该突变株值得进一步研究。
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| 关 键 词： | 透明质酸产生菌 诱变 培养基 优化 |
| 文章编号： | 1006-8376（2008）01-0037-04 |
| 收稿时间： | 2007-11-02 |
| 修稿时间： | 2007年11月2日 |
Studies on Mutation and Medium Composition of Hyaluronic Acid Producer |
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| WU Ming-xia,DENG Jing,WU Hua-chang,Wei Dai-xu,Chen Yi-de.Studies on Mutation and Medium Composition of Hyaluronic Acid Producer[J].Amino Acids & Biotic Resources,2008,30(1):37-40. |
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| Authors: | WU Ming-xia DENG Jing WU Hua-chang Wei Dai-xu Chen Yi-de |
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| Abstract: | Primary researches on the mutant screening of hyaluronic acid(HA) producer were studied.A strain which can secrete HA greatly was got.The optimum compositions of the substrate through orthogonal experiment were as follows: 2% starch,1% yeast extract,1% beef extract,1%peptone and 0.1% NaHCO3.The maximum yield of HA was 4.59g·L-1 and 1.29 times of the start substrate. |
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| Keywords: | hyaluronic acid(HA) producer screening substrate composition optimization |
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